sucrose- and h+-dependent charge movements associated with the gating of sucrose transporter zmsut1蔗糖-和h +端依赖电荷运动与蔗糖运输zmsut1的闸门.pdfVIP

+ Sucrose- and H -Dependent Charge Movements Associated with the Gating of Sucrose Transporter ZmSUT1 1 2 3 4 4 Armando Carpaneto , Hermann Koepsell , Ernst Bamberg , Rainer Hedrich , Dietmar Geiger * ¨ ¨ ¨ ¨ 1 Istituto di Biofisica-CNR, Genova, Italy, 2 Institut fur Anatomie und Zellbiologie, Universitat Wurzburg, Wurzburg, Germany, 3 Max-Plank-Institute for Biophysics, Frankfurt ¨ ¨ ¨ am Main, Germany, 4 Julius-von-Sachs-Institut, Molekulare Pflanzenphysiologie und Biophysik, Universitat Wurzburg, Wurzburg, Germany Abstract Background: In contrast to man the majority of higher plants use sucrose as mobile carbohydrate. Accordingly proton- driven sucrose transporters are crucial for cell-to-cell and long-distance distribution within the plant body. Generally very negative plant membrane potentials and the ability to accumulate sucrose quantities of more than 1 M document that plants must have evolved transporters with unique structural and functional features. Methodology/Principal Findings: To unravel the functional properties of one specific high capacity plasma membrane sucrose transporter in detail, we expressed the sucrose/H+ co-transporter from maize ZmSUT1 in Xenopus oocytes. Application of sucrose in an acidic pH environment elicited inward proton currents. Interestingly the sucrose-dependent H+ transport was associated with a decrease in membrane capacitance (Cm). In addition to sucrose Cm was modulated by the membrane po