sweepoviruses cause disease in sweet potato and related ipomoea spp. fulfilling kochs postulates for a divergent group in the genus begomovirussweepoviruses引起疾病的红薯和相关的番薯spp。实现科赫法则属begomovirus发散集团.pdfVIP

Sweepoviruses Cause Disease in Sweet Potato and Related Ipomoea spp.: Fulfilling Koch’s Postulates for a Divergent Group in the Genus Begomovirus . ´ . ´ ´ ´ ´ Helena P. Trenado , Anelise F. Orılio , Belen Marquez-Martın, Enrique Moriones, Jesus Navas-Castillo* ´ ´ ´ Instituto de Hortofruticultura Subtropical y Mediterranea ‘‘La Mayora’’ (IHSM), Consejo Superior de Investigaciones Cientıficas, Algarrobo-Costa, Malaga, Spain Abstract Sweet potato (Ipomoea batatas) and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae), known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch’s postulates have not been fullfilled for any of the viruses in this group. Three novel species of sweepoviruses have recently been described in Spain: Sweet potato leaf curl Lanzarote virus (SPLCLaV), Sweet potato leaf curl Spain virus (SPLCSV) and Sweet potato leaf curl Canary virus (SPLCCaV). Here we describe the generation of the first infectious clone of an isolate (ES:MAL:BG30:06) of SPLCLaV. The clone consisted of a complete tandem dimeric viral genome in a binary vector. Successful infection by agroinoculation of several species of Ipomoea (including sweet potato) and Nicotiana benthamiana was confirmed by PCR, dot blot and Southern blot hybridization. Symptoms observed in infected plants consisted of leaf curl, yellowing, growth reduction and vein yello