synthesis and characterization of cationic glycidyl-based poly(aminoester)-folic acid targeting conjugates and study on gene delivery合成和表征的阳离子glycidyl-based保利(aminoester)叶酸酸研究针对轭合物和基因传递.pdfVIP

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synthesis and characterization of cationic glycidyl-based poly(aminoester)-folic acid targeting conjugates and study on gene delivery合成和表征的阳离子glycidyl-based保利(aminoester)叶酸酸研究针对轭合物和基因传递.pdf

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synthesis and characterization of cationic glycidyl-based poly(aminoester)-folic acid targeting conjugates and study on gene delivery合成和表征的阳离子glycidyl-based保利(aminoester)叶酸酸研究针对轭合物和基因传递

Molecules 2012, 17, 9056-9069; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery Pai Feng Tsai 1, Wei Yang Chang 2, Yu Che Hsiao 3, Kuo Jung Li 2 and Min Da Shau 2,* 1 Department of Occupational Safety and Hygiene, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec. 1, Jen-Te, Taiwan; E-Mail: paifeng@.tw 2 Department of Biotechnology, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec. 1, Jen-Te, Taiwan; E-Mails: oz.j.w.y@ (W.Y.C.); dragonli@.tw (K.J.L.) 3 Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996, USA; E-Mail: k7310162002@ * Author to whom correspondence should be addressed; E-Mail: minda.shau@; Tel.: +886-6-266-4911 (ext. 2510); Fax: +886-6-266-2135. Received: 24 May 2012; in revised form: 18 July 2012 / Accepted: 21 July 2012 / Published: 30 July 2012 Abstract: A new poly(aminoester) (EPAE-FA) containing folic acid and amino groups in the backbone and side chain was synthesized. EPAE-FA self-assembled readily with the plasmid DNA (pCMV-βgal) in HEPES buffer and was characterized by dynamic light scattering, zeta potential, fluorescence images, and XTT cell viability assays. To evaluate the transfection effect of graft ratio of FA on the EPAE system, EPAE-FA polymers with two different graft ratios (EPAE-FA12k and EPAE-FA14k) were also prepared. This study