testing bias in clinical databases methodological considerations临床数据库方法论考虑测试的偏见.pdfVIP

Velthove et al. Emerging Themes in Epidemiology 2010, 7:2 EMERGING THEMES /content/7/1/2 IN EPIDEMIOLOGY M E T H O D O L O G Y Open Access Methodology Testing bias in clinical databases: methodological considerations 1,2 1 1 3 1,2 Karin J Velthove , Hubert GM Leufkens* , Patrick C Souverein , René C Schweizer and Wouter W van Solinge Abstract Background: Laboratory testing in clinical practice is never a random process. In this study we evaluated testing bias for neutrophil counts in clinical practice by using results from requested and non-requested hematological blood tests. Methods: This study was conducted using data from the Utrecht Patient Oriented Database. This clinical database is unique, as it contains physician requested data, but also data that are not requested by the physician, but measured as result of requesting other hematological parameters. We identified adult patients, hospitalized in 2005 with at least two blood tests during admission, where requests for general blood profiles and specifically for neutrophil counts were contrasted in scenario analyses. Possible effect modifiers were diagnosis and glucocorticoid use. Results: A total of 567 patients with requested neutrophil counts and 1,439 patients with non-requested neutrophil counts were analyzed. The absolute neutrophil count at admission differed with a mean of 7.4 × 109/l for requested counts and 8.3 × 109/l for non-requested counts (p-value 0.001). This difference could be explained for 83.2% by the occurrence of cardiovascular disease as underlying disease and