testing human sperm chemotaxis how to detect biased motion in population assays测试人类精子趋化作用如何检测偏向运动人口分析.pdfVIP

Testing Human Sperm Chemotaxis: How to Detect Biased Motion in Population Assays 1 1 1 2,3 Leah Armon , S. Roy Caplan , Michael Eisenbach , Benjamin M. Friedrich * 1 Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel, 2 Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot, Israel, 3 Max Planck Institute for the Physics of Complex Systems, Dresden, Germany Abstract Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming. Citation: Armon L, Caplan SR, Eisenbach M, Friedrich BM (2012) Testing Human Sperm Chemotaxis: How to Detect Biased Motion in Population Assays. PLoS ONE 7(3): e32909. doi:10.1371/journal.pone.0032909 Editor: Enrico Scalas, Universita’ del Piemonte Orientale, Italy Received June 29, 2011; Accepted February 7, 2012; Published March 8, 2012 Copyright: 2012 Armon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The author Be