the absence of cd47 promotes nerve fiber growth from cultured ventral mesencephalic dopamine neurons没有cd47促进神经纤维生长培养腹侧中脑多巴胺神经元.pdfVIP

The Absence of CD47 Promotes Nerve Fiber Growth from Cultured Ventral Mesencephalic Dopamine Neurons 1 1 2 1 ¨ 1 Franziska Marschinke , Sanaz Hashemian , Takashi Matozaki , Per-Arne Oldenborg , Ingrid Stromberg * ˚ ˚ 1 Department of Integrative Medical Biology, Umea University, Umea, Sweden, 2 Division of Molecular and Cellular Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan Abstract In ventral mesencephalic organotypic tissue cultures, two timely separated sequences of nerve fiber growth have been observed. The first appearing nerve fiber pattern is a long-distance outgrowth that occurs before astrocytes start to proliferate and migrate to form an astrocytic monolayer that finally surrounds the tissue slice. These long-distance growing nerve fibers are retracted as the astrocytes migrate, and are followed by a secondary outgrowth. The secondary outgrowth is persistent in time but reaches short distances, comparable with outgrowth seen from a dopaminergic graft implanted to the brain. The present study was focused on the interaction between the astrocytes and the long-distance growing non- glial associated nerve fibers. Cross talk between astroglia and neurite formation might occur through the integrin- associated protein CD47. CD47 serves as a ligand for signal regulatory protein (SIRP) a and as a receptor for the extracellular matrix protein thrombospondin-1 (TSP-1). Embryonic day 14 ventral mesencephalic tissue from CD47+/+ and CD472/ 2 mice was used to investigate astrocytic migration and the tyrosine hydroxylase (TH) –posi