the antiviral drug valacyclovir successfully suppresses salivary gland hypertrophy virus (sghv) in laboratory colonies of glossina pallidipes抗病毒药物valacyclovir成功抑制唾腺肥大的病毒在实验室殖民地(sghv)舌蝇pallidipes.pdfVIP

The Antiviral Drug Valacyclovir Successfully Suppresses Salivary Gland Hypertrophy Virus (SGHV) in Laboratory Colonies of Glossina pallidipes 1 1 1 1 2 Adly M.M. Abd-Alla *, Henry Adun , Andrew G. Parker , Marc J.B. Vreysen , Max Bergoin ´ 1 Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria, 2 Laboratoire de Pathologie Comparee, ´ Universite Montpellier, Montpellier, France Abstract Many species of tsetse flies are infected with a virus that causes salivary gland hypertrophy (SGH) symptoms associated with a reduced fecundity and fertility. A high prevalence of SGH has been correlated with the collapse of two laboratory colonies of Glossina pallidipes and colony maintenance problems in a mass rearing facility in Ethiopia. Mass-production of G. pallidipes is crucial for programs of tsetse control including the sterile insect technique (SIT), and therefore requires a management strategy for this virus. Based on the homology of DNA polymerase between salivary gland hypertrophy virus and herpes viruses at the amino acid level, two antiviral drugs, valacyclovir and acyclovir, classically used against herpes viruses were selected and tested for their toxicity on tsetse flies and their impact on virus replication. While long term per os administration of acyclovir resulted in a significant reduction of productivity of the colonies, no negative effect was observed in colonies fed with valacyclovir-treated blood. Furthermore, treatment of a tsetse co