the characterization of helicobacter pylori dna associated with ancient human remains recovered from a canadian glacier幽门螺杆菌dna的特征与古代人类遗骸从加拿大冰川中恢复过来.pdfVIP

The Characterization of Helicobacter pylori DNA Associated with Ancient Human Remains Recovered from a Canadian Glacier 1 2 2 1 Treena Swanston *, Monique Haakensen , Harry Deneer , Ernest G. Walker 1 Department of Archaeology and Anthropology, University of Saskatchewan, Saskatoon, Canada, 2 Department of Pathology and Laboratory Medicine, University of Saskatchewan, Saskatoon, Canada Abstract Helicobacter pylori is a gram-negative bacterium that colonizes the stomach of nearly half of the world’s population. Genotypic characterization of H. pylori strains involves the analysis of virulence-associated genes, such as vacA, which has multiple alleles. Previous phylogenetic analyses have revealed a connection between modern H. pylori strains and the ¨ movement of ancient human populations. In this study, H. pylori DNA was amplified from the stomach tissue of the Kwaday ¨ ` Dan Ts’ınchi individual. This ancient individual was recovered from the Samuel Glacier in Tatshenshini-Alsek Park, British Columbia, Canada on the traditional territory of the Champagne and Aishihik First Nations and radiocarbon dated to a timeframe of approximately AD 1670 to 1850. This is the first ancient H. pylori strain to be characterized with vacA sequence data. The Tatshenshini H. pylori strain has a potential hybrid vacA m2a/m1d middle (m) region allele and a vacA s2 signal (s) region allele. A vacA s2 allele is more commonly identified with Western strains, and this s