the comparison between circadian oscillators in mouse liver and pituitary gland reveals different integration of feeding and light schedules昼夜振荡器之间的比较小鼠肝脏和脑垂体显示不同的喂养和集成光时间表.pdfVIP

The Comparison between Circadian Oscillators in Mouse Liver and Pituitary Gland Reveals Different Integration of Feeding and Light Schedules Isabelle M. Bur1,2,3¤, Sonia Zouaoui1,2,3, Pierre Fontanaud1,2,3, Nathalie Coutry1,2,3, Franc¸ois Molino1,2,3, ` 1,2,3, Patrice Mollard1,2,3, Xavier Bonnefont1,2,3* Agnes O. Martin ´ ´ 1 CNRS, UMR 5203, Institut de Genomique Fonctionnelle, Montpellier, France, 2 INSERM, U661, Montpellier, France, 3 Universite Montpellier, Montpellier, France Abstract The mammalian circadian system is composed of multiple peripheral clocks that are synchronized by a central pacemaker in the suprachiasmatic nuclei of the hypothalamus. This system keeps track of the external world rhythms through entrainment by various time cues, such as the light-dark cycle and the feeding schedule. Alterations of photoperiod and meal time modulate the phase coupling between central and peripheral oscillators. In this study, we used real-time quantitative PCR to assess circadian clock gene expression in the liver and pituitary gland from mice raised under various photoperiods, or under a temporal restricted feeding protocol. Our results revealed unexpected differences between both organs. Whereas the liver oscillator always tracked meal time, the pituitary circadian clockwork showed an intermediate response, in between entrainment by the light regimen and the feeding-fasting rhythm. The same composite response was also observed in the pituitary gland from adrenalectomized mice under daytime restricted feeding, suggesting that circulating glucocorticoids do not inhibit full entrainment of the pituitary clockwork by meal tim