the genomic diversity and phylogenetic relationship in the family iridoviridae基因多样性和系统发育关系的家庭iridoviridae.pdfVIP

Viruses 2010, 2, 1458-1475; doi:10.3390/v2071458 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article The Genomic Diversity and Phylogenetic Relationship in the Family Iridoviridae Heather E. Eaton, Brooke A. Ring and Craig R. Brunetti * Trent University, Peterborough, ON, K9J 7B8, Canada, E-Mails: heathereaton@trentu.ca (H.E.E.); brookering@trentu.ca (B.A.R.) * Author to whom correspondence should be addressed; E-Mail: craigbrunetti@trentu.ca; Tel.: +1-705-748-1011; Fax: +1-705-748-1205. Received: 8 June 2010; in revised form: 12 July 2010 / Accepted: 13 July 2010 / Published: 15 July 2010 Abstract: The Iridoviridae family are large viruses (~120-200 nm) that contain a linear double-stranded DNA genome. The genomic size of Iridoviridae family members range from 105,903 bases encoding 97 open reading frames (ORFs) for frog virus 3 to 212,482 bases encoding 211 ORFs for Chilo iridescent virus. The family Iridoviridae is currently subdivided into five genera: Chloriridovirus, Iridovirus, Lymphocystivirus, Megalocytivirus, and Ranavirus . Iridoviruses have been found to infect invertebrates and poikilothermic vertebrates, including amphibians, reptiles, and fish. With such a diverse array of hosts, there is great diversity in gene content between different genera. To understand the origin of iridoviruses, we explored the phylogenetic relationship between individual iridoviruses and defined