the global transcriptional response of fission yeast to hydrogen sulfide全球转录反应裂殖酵母硫化氢.pdfVIP

The Global Transcriptional Response of Fission Yeast to Hydrogen Sulfide 1,2 1 1,2 1,2 Xu Jia , Weizhi He , Alastair I. H. Murchie *, Dongrong Chen * 1 Institute of Biomedical Science, Fudan University, Shanghai, China, 2 School of Pharmacy, Fudan University, Pudong, Shanghai, China Abstract Background: Hydrogen sulfide (H S) is a newly identified member of the small family of gasotransmitters that are 2 endogenous gaseous signaling molecules that have a fundamental role in human biology and disease. Although it is a relatively recent discovery and the mechanism of H2S activity is not completely understood, it is known to be involved in a number of cellular processes; H2S can affect ion channels, transcription factors and protein kinases in mammals. Methodology/Principal Findings: In this paper, we have used fission yeast as a model organism to study the global gene expression profile in response to H2S by microarray. We initially measured the genome-wide transcriptional response of fission yeast to H S. Through the functional classification of genes whose expression profile changed in response to H S, we 2 2 found that H2S mainly influences genes that encode putative or known stress proteins, membrane transporters, cell cycle/ meiotic proteins, transcription factors and respiration protein in the mitochondrion. Our analysis showed that there was a significant overlap between the genes affected by H2S and the stress response. We identified that the target genes of the MAPK pathway respond to H