the impact of the absence of aliphatic glucosinolates on insect herbivory in arabidopsis没有脂肪族硫配糖体的影响在拟南芥昆虫取食.pdfVIP

The Impact of the Absence of Aliphatic Glucosinolates on Insect Herbivory in Arabidopsis 1. 2. 3 4 4 Jules Beekwilder *, Wessel van Leeuwen , Nicole M. van Dam , Monica Bertossi , Valentina Grandi , 5 6 7 1 1 1 Luca Mizzi , Mikhail Soloviev , Laszlo Szabados , Jos W. Molthoff , Bert Schipper , Hans Verbocht , 1 4 2 1 Ric C. H. de Vos , Piero Morandini , Mark G. M. Aarts , Arnaud Bovy 1 Plant Research International, Wageningen, The Netherlands, 2 Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands, 3 Netherlands Institute of Ecology (NIOO-KNAW), Heteren, The Netherlands, 4 Department of Biology, University of Milan, CNR Biophysics Institute, Milano, Italy, 5 Department of Biomolecular Sciences and Biotechnology, University of Milan, CNR Biophysics Institute, Milano, Italy, 6 School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom, 7 Biological Research Center, Szeged, Hungary Abstract Aliphatic glucosinolates are compounds which occur in high concentrations in Arabidopsis thaliana and other Brassicaceae species. They are important for the resistance of the plant to pest insects. Previously, the biosynthesis of these compounds was shown to be regulated by transcription factors MYB28 and MYB29. We now show that MYB28 and MYB29 are partially redundant, but in the absence of both, the synthesis of all aliphatic glucosinolates is blocked. Untargeted and targeted biochemical analyses of leaf metabolites sho