diagnosis of pancreatic ductal adenocarcinoma and chronic pancreatitis by measurement of microrna abundance in blood and tissue胰腺导管腺癌的诊断和测量慢性胰腺炎的microrna在血液和组织丰富.pdfVIP

Diagnosis of Pancreatic Ductal Adenocarcinoma and Chronic Pancreatitis by Measurement of microRNA Abundance in Blood and Tissue 1. 2. 3 3 1 2 Andrea S. Bauer , Andreas Keller , Eithne Costello , William Greenhalf , Melanie Bier , Anne Borries , 2 3 ¨ 4 4 4 ¨ 1 Markus Beier , John Neoptolemos , Markus Buchler , Jens Werner , Nathalia Giese , Jorg D. Hoheisel * 1 Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany, 2 Biomarker Discovery Center Heidelberg, Heidelberg, Germany, 3 The National Institute for Health Research Pancreas Biomedical Research Unit and the Liverpool Experimental Cancer Medicine Center, Royal Liverpool University Hospital, Liverpool, United Kingdom, 4 Department of Surgery, University of Heidelberg, Heidelberg, Germany Abstract A solid process for diagnosis could have a substantial impact on the successful treatment of pancreatic cancer, for which currently mortality is nearly identical to incidence. Variations in the abundance of all microRNA molecules from peripheral blood cells and pancreas tissues were analyzed on microarrays and in part validated by real-time PCR assays. In total, 245 samples from two clinical centers were studied that were obtained from patients with pancreatic ductal adenocarcinoma or chronic pancreatitis and from healthy donors. Utilizing the minimally invasive blood test, receiver operating characteristic (ROC) curves and the corresponding area under the curve (AUC) analysis demonstrated very high sensitivity and specificity of a distinction between healthy people and patients with