dmso and betaine greatly improve amplification of gc-rich constructs in de novo synthesisdmso和甜菜碱大大提高放大gc-rich构造的从头合成.pdfVIP

DMSO and Betaine Greatly Improve Amplification of GC- Rich Constructs in De Novo Synthesis Michael A. Jensen*, Marilyn Fukushima, Ronald W. Davis Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America Abstract In Synthetic Biology, de novo synthesis of GC-rich constructs poses a major challenge because of secondary structure formation and mispriming. While there are many web-based tools for codon optimizing difficult regions, no method currently exists that allows for potentially phenotypically i