drosophila melanogaster dhcf interacts with both pcg and trxg epigenetic regulators黑腹果蝇dhcf与pcg和trxg后生监管机构.pdfVIP

Drosophila melanogaster dHCF Interacts with both PcG and TrxG Epigenetic Regulators 1 2 1 Sara Rodriguez-Jato , Ana Busturia , Winship Herr * ´ ´ 1 Center for Integrative Genomics, University of Lausanne, Genopode, Lausanne, Switzerland, 2 Centro de Biologıa Molecular Severo Ochoa, Madrid, Spain Abstract Repression and activation of gene transcription involves multiprotein complexes that modify chromatin structure. The integration of these complexes at regulatory sites can be assisted by co-factors that link them to DNA-bound transcriptional regulators. In humans, one such co-factor is the herpes simplex virus host-cell factor 1 (HCF-1), which is implicated in both activation and repression of transcription. We show here that disruption of the gene encoding the Drosophila melanogaster homolog of HCF-1, dHCF, leads to a pleiotropic phenotype involving lethality, sterility, small size, apoptosis, and morphological defects. In Drosophila, repressed and activated transcriptional states of cell fate-determining genes are maintained throughout development by Polycomb Group (PcG) and Trithorax Group (TrxG) genes, respectively. dHCF mutant flies display morphological phenotypes typical of TrxG mutants and dHCF interacts genetically with both PcG and TrxG genes. Thus, dHCF inactivation enhances the mutant phenotypes of the Pc PcG as well as brm and mor TrxG genes, suggesting that dHCF possesses Enhancer of TrxG and PcG (ETP) properties. Additionally, dHCF interacts with the previously esta