engineered picornavirus vpg-rna substrates analysis of a tyrosyl-rna phosphodiesterase activity设计小核糖核酸病毒vpg-rna基质分析tyrosyl-rna磷酸二酯酶的活动.pdfVIP

Engineered Picornavirus VPg-RNA Substrates: Analysis of a Tyrosyl-RNA Phosphodiesterase Activity Janet M. Rozovics, Richard Virgen-Slane, Bert L. Semler* Department of Microbiology and Molecular Genetics, School of Medicine, University of California Irvine, Irvine, California, United States of America Abstract Using poliovirus, the prototypic member of Picornaviridae, we have further characterized a host cell enzymatic activity found in uninfected cells, termed ‘‘unlinkase,’’ that recognizes and cleaves the unique 5 9 tyrosyl-RNA phosphodiester bond fo