薄层扫描法测定消积片中盐酸小檗碱的含量（Determination of berberine hydrochloride in tablets by TLC scanning）.docVIP

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薄层扫描法测定消积片中盐酸小檗碱的含量（Determination of berberine hydrochloride in tablets by TLC scanning）.doc

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薄层扫描法测定消积片中盐酸小檗碱的含量（Determination of berberine hydrochloride in tablets by TLC scanning）

薄层扫描法测定消积片中盐酸小檗碱的含量（Determination of berberine hydrochloride in tablets by TLC scanning） Determination of berberine hydrochloride in tablets by TLC scanning Update Date: 2011-08-06 hits: Wei Bo on behalf of snow Ping, Liu Pingxiang CLC number: R927.2, document identification code: B Article number:(2000) -The product is prepared from 12 common Chinese herbs, such as ginseng, Atractylodes macrocephala, licorice root, Coptis chinensis and dried tangerine peel. In order to press and correct the taste, a large amount of lactose and sucrose and other additives were added into the prescription. The presence of these components affected the content of berberine hydrochloride. In the past, alumina column chromatography ([1]) was used to remove impurities, but there was still more sugar in the sample solution, which caused the poor separation of the thin-layer chromatography and influenced the scanning determination. In this paper, macroporous resin adsorption method is used to solve this problem. 1 experimental materials 1.1 instrument Swiss CAMAG II thin layer chromatography scanner (version 3.15 data processing software). 1.2 drugs and reagents The product was provided by the Xinxiang peoples pharmaceutical factory in Henan. The reference standard of berberine chloride was provided by the China Institute of pharmaceutical and biological products (batch number 713-9204), and the purity met the requirements of the content determination. 2 determination method 2.1 scan condition Using 3% sodium carboxymethyl cellulose as the silica gel G thin plate adhesive, with benzene - ethyl acetate - methanol isopropanol concentrated ammonia solution (12: 6: 3: 3: 1) as the agent, before the commencement of an equal volume of concentrated ammonia solution to pre equilibrium 15 minutes; scanning wavelength: 350nm s. 2.2 solution preparation 2.2. 1 contrast solution: take the berberine hydrochloride accurately, the amount of the standard amount, and add ethanol, each 1