补骨脂素加长波紫外线对NB4, K562白血病细胞株作用.doc

补骨脂素加长波紫外线对NB4, K562白血病细胞株作用 　 作者：向阳，葛永亮，张德杰，陈楠楠，黄世林 【关键词】 紫外线 Effects of psoralen plus ultraviolet A on NB4 and K562 leukemia cell lines 　　【Abstract】 AIM: To explore the effects of psoralen plus ultraviolet A（PUVA）on NB4 cells and K562 cells. METHODS: NB4 cells and K562 cells were subjected to the research.The effects of psoralen extracted from Chinese medicine plus ultraviolet A(UVA) of wave length 360 nm on leukemia cell lines were observed. The factorial design was applied to observe the effects of psoralen at different concentrations, UVA with different exposure time, on the inhibition rates and apoptosis rates of different leukemia cell lines, and the interaction among the each factor. RESULTS: ①When the concentration of psoralen was 80 mg/L and the exposure time of UVA was 15 min，the inhibition rates of NB4 cells reached the peak. When the concentration of psoralen was 80 mg/L and the exposure time of UVA was 10 min, the inhibition rates of K562 cells reached the peak. ②When the concentration of psoralen was 40 mg/L and exposure tim of UVA was 10 min，the apoptosis rates of NB4 cells reached the peak. When the concentration of psoralen was 80 mg/L and exposure time of UVA was 15 min，the apoptosis rates of K562 cells reached the peak. ③The inhibition rates and the apoptosis rates of different leukemia cell lines induced by psoralen at different concentrations plus UVA with different exposure time had statistical differences, and there was the interaction between each factor. CONCLUSION: PUVA can inhibit the growth of NB4 and K562 cell lines and induce their apoptosis. The most effective point of PUVA on NB4 and K562 cell lines is that the concentration of psoralen is 40-80 mg/L and the exposure time of UVA is 10-15 min. 　　【Keywords】 PUVA therapy; ultraviolet rays; NB4 cell line; K562 cell line 　　【摘要】 目的：探讨补骨脂素(PSO)加长波紫外线(UVA)对NB4，K562白血病细胞株的作用. 方法：以NB4，K562细胞株为研究对象，观察中药补骨脂中提取的PSO加波长为360 nm的UVA对人白血病细胞株的作用；采用析因设计，观察不同浓度的PSO、不同照射时间的UVA对不同白血病细胞株抑制率、凋亡率的影响以及各因素间的交