表皮葡萄球菌全面调节子表达在生物被膜耐药性中作用.doc

表皮葡萄球菌全面调节子表达在生物被膜耐药性中作用 　 作者：贾宁，徐志凯，袁云娥，索继江，邢玉斌 【关键词】 表皮葡萄球菌；,生物膜；,实时定量;,逆转录聚合酶链反应 　　Relationship between expressions of global regulators and erythromycin resistance of Staphylococcus epidermidis biofilm 　　【Abstract】 AIM: To explore the relationship between the expressions of global regulators and the erythromycin resistance of Staphylococcus epidermidis (S. epidermidis) biofilm. METHODS: The expressions of global regulators (agr, sarA and sigB) in biofilm cells of S. epidermidis 1457 with and without erythromycin resistance were detected by SYBR green I realtime RTPCR. RESULTS: The expressions of agrD and RNAIII were significantly higher in biofilm cells than those in planktonic cells in both erythromycin and nonerythromycin groups （Plt;0.05）. The expression of sarA in biofilm cells was significantly higher than that in planktonic cells, but there was no significant difference in biofilm cells between erythromycin group and nonerythromycin group. The expression of sigB and its active regulation gene asp23 in biofilm cells showed no significant difference between erythromycin and nonerythromycin groups (Pgt;0.05). CONCLUSION: agrD and RNAIII take part in quorumsensing regulation, which suggests that quorumsensing system plays an important role in erythromycin resistance of S. epidermidis biofilm. 　　【Keywords】 Staphylococcus epidermidis; biofilm; realtime quantitative; RTPCR 　　【摘要】 目的：探讨表皮葡萄球菌全面调节子的表达在生物被膜耐药性中的作用. 方法：采用实时荧光定量RTPCR对表皮葡葡球菌1457生物被膜内细菌红霉素作用前后全面调节子(agr, sarA, sigB)的表达进行检测. 结果：在表皮葡萄球菌1457中，agrD和RNAⅢ在膜内菌中的表达均高于浮游菌，且红霉素作用24 h后的表达高于作用前（Plt;0.05）；sarA膜内菌的表达高于浮游菌（Plt;0.05），但红霉素作用前后的表达无统计学差异（Pgt;0.05）；膜内菌sigB和反应sigB 调控的活性基因asp23的表达红霉素作用前后无统计学差异（Pgt;0.05）. 结论：由于agrD和RNAⅢ参与群体效应系统的调节，提示群体效应调节在红霉素耐药性增加中起着重要作用. 　　【关键词】 表皮葡萄球菌；生物膜；实时定量;逆转录聚合酶链反应 　　0　引言 　　表皮葡萄球菌吸附于机体粘膜或插管、人工关节等生物医学材料表面，分泌多糖基质、纤维蛋白、脂多糖等多糖复合物，相互粘连并克隆产生生物被膜. 生物被膜的产生使膜内菌对抗生素的耐药性比浮游菌增加10~1000倍, 其引发的感染常常只有移走植入物才能控制［1］. 生物膜内细菌如何形成对抗生素的耐药性，目前还不十分清楚，相关的研究报道较少. 不同的生物被膜的组成和不同的抗生素，其耐药机制也不相同［2-3］. 我们采用实时荧光定量RTPCR