调脂康胶囊不同提取部位对高血脂大鼠脂质水平影响.docVIP

调脂康胶囊不同提取部位对高血脂大鼠脂质水平影响 　作者：姚红霞,王亮,杨超燕,韦燕萍,唐春萍 【摘要】 目的 探讨调脂康胶囊对高血脂大鼠脂质水平影响的有效部位。方法 采用饮食性高血脂大鼠模型，用极性由小到大的3种有机溶剂和水依次萃取调脂康胶囊，所得各提取部位组分别给大鼠ig给药，测定血清三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)和肝脏TG、TC的变化。结果 大鼠灌高脂乳剂4周后，模型组大鼠血清TC、TG、LDLC均较正常组明显升高，肝中TC、TG均明显升高;给药4周后，调脂康胶囊氯仿提取部位组、乙酸乙酯提取部位组、正丁醇提取部位组、水部位组、全方组均能显著降低血清TC、LDLC水平(Plt;0.05或Plt;0.01);正丁醇提取部位组、全方组能显著降低血清TG水平;正丁醇提取部位组能显著降低TC、TG、LDLC，且与全方比较差异无显著性 (Pgt;0.05);正丁醇提取部位组、水部位组、全方组与模型组比较，肝中TC明显降低(Plt;0.05或Plt;0.01)，乙酸乙酯提取部位组、全方组与模型组比较，肝中TG明显降低(Plt;0.05)。结论 调脂康胶囊乙酸乙酯和正丁醇提取物对高血脂大鼠有降脂作用。 【关键词】 调脂康胶囊;高血脂症模型;有效部位 )Abstract:Objective To study lipidlowering effects of extractions of Tiaozhikang capsules extracted with chloroform,acetic ether,nbutyl alcohol,water.Methods Extractions of Tiaozhikang capsules were extracted with four organic solvents in sequence according to their polarities from higher to lower. Then the extractions were intragastrically administered to dietinduced hyperlipidemic rats,and changes in serum triacylglycerol level (TG),serum total cholesterol level (TC),serum high density lipoprotein cholesterol level (HDLC),low density lipoprotein cholesterol level (LDLC),liver triacylglycerol level,and liver total cholesterol level were measured.Results 72 Sprague Dawley (SD) rats were administered with high fat emulsion for four weeks,and serum TG level,serum TC level,serum LDLC level,liver TC level,and liver TG level were higher than those in the control group. Serum TC levels and serum LDLC level in the groups administered intragastrically with extractions of Tiaozhikang capsules respectively extracted with chloroform,acetic ether,nbutyl alcohol,water,and Tiaozhikang capsules decreased significantly (Plt;0.05 or Plt;0.01); serum TG levels in the groups intragastrically administered with extractions ofTiaozhikang capsules extracted with nbutyl alcohol and Tiaozhikang capsules decreased significantly; serum TC level,serum TG level,serum LDLC level decreased significantly in the group intrastrically