调节蛋白表达影响.docVIP

调节蛋白表达影响 　 作者：潘曦明 潘景业 张近波 陈洁 【摘要】 目的 探讨失血性休克大鼠TM、TM mRNA的变化机制及参附的干预作用的研究。 方法 SD大鼠分成：假休克组，休克组，林格液复苏组，参附复苏组。休克及复苏2h 后，处死大鼠取出肝脏, Western blotting法测TM 变化，用RT－PCR法测TM mRNA的变化。结果 假休克组有 TM 、TM mRNA表达。休克组、林格液复苏组TM 表达下降，TM mRNA表达上调，与假休克组比较有显著统计学意义。参附复苏组TM 表达上调，TM mRNA 表达显著下调恢复，与假休克组比较无统计学意义，与休克组、林格液复苏组比较有显著统计学意义。结论 失血性休克TM 蛋白表达下降，TM mRNA表达上调，与休克损伤有关。林格液对蛋白C激活系统表达无明显调节作用。参附通过对内皮细胞的保护作用，具有抗休克作用。 【关键词】 失血性休克 凝血酶调节蛋白 参附注射液 【Abstract】 Objective To investigate the machenism of thrombomodulin (TM) TM mRNA changes in rats with hemorrhagic shock and the effect of Shenfu injection on them. Methods SD rats were assigned to the following four groups: sham shock; HS group; RS group; SF group. After start resuscitate for 2 hours ，the liver of rats were taken out and thrombomodulin was performed with Western blotting, The mRNA expression in liver of thrombomodulin was detected by reverse-transcription polymerase chain reaction(RT－PCR). Results The level of TM in liver obviously decreased in HS group, RS group, the mRNA expression of TM increased in HS group, RS group. Furthermore the level of TM in liver in SF group were significantly higher than that in HS group and RS group, the mRNA expression of TM in SF group was significantly lower than that in HS group or RS group. Conclusion The mRNA expression level of TM were significantly up-regulated, and expression of TM was down-regulated in liver of rats, which may be caused by the injury of hemorrhagic shock. Ringer’s solution has no effect on the protein C activation complex in hemorrhagic shock. Shenfu injection has an anti-hemorrhagic shock effect by protecting endothelial cell from injury . 【Key Words】 Hemorrhagic shock Thrombomodulin Shenfu injection 　　创伤死亡的首要原因是失血性休克。2007年欧洲重症监护医学协会、欧洲休克协会等专家发表《控制大创伤后出血的治疗指南》，指出大出血患者常由血管损伤及凝血功能异常所致。血管内皮细胞的主要功能为抗血栓形成,这也是维持血液流动性的重要机制之一。PC系统发挥抗凝作用与血管内皮细胞上的凝血酶调节蛋白(thrombomodulin, TM) 、内皮细胞蛋白C 受体(endothelial protein C receptor, EPCR) 及血浆中的蛋白C(protein C,PC) 等有关。该系统是凝血酶生成后对凝血系统活化有负反馈作用的一个调节系统，不仅具有抗凝作用，而且在