大鲵低聚糖肽安全性毒理学初步研究.docVIP

大鲵低聚糖肽安全性的毒理学初步研究 姜万维1, 余睿智2, 曲 敏2, 赵前程2, 王庆辉1, 佟长青2, 李 伟2* (1. 大连大学附属中山医院麻醉二科, 大连 116001; 2. 大连海洋大学食品科学与工程学院, 大连 116023) 摘 要: 目的 30 d喂养实验, 评价大鲵低聚糖肽的安全性。结果 雌、雄性小鼠急性经口在11764.7 mg/kg·BW剂量时, 未见毒性不良反应, 根据急性毒性剂量分级标准, 大鲵低聚糖肽属于无毒级; 大鲵低聚糖肽无致小鼠骨髓细胞微核效应, 30 d喂养实验未见大鼠的生长发育、生物化学指标、脏器/体质量比值比及病理学变化。结论 毒性实验未见大鲵低聚糖肽产生明显毒副作用。 关键词 Toxicology of glycopeptides from Andrias davidianus JIANG Wan-Wei1, YU Rui-Zhi2, QU Min2, ZHAO Qian-Cheng2, WANG Qing-Hui1,TONG Chang-Qing2, LI Wei2* (1. Second Anesthesia Department, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China;2. College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China) ABSTRACT: Objective To investigate the safety of glycopeptides from Andrias davidianus. Methods The safety of glycopeptides from A. davidianus was evaluated by the acute oral toxicity test of mice, the micronucleus calculation of mouse bone marrow cells, and 30 d feeding test of rats. Results No toxicity was found when acute oral toxicity dose was 11764.7 mg/kg·BW in ICR mice (female and male). Glycopeptides from A. davidianus could be categorized as non-toxic according to the acute toxicity dose classification standard. The micronucleus calculation of mouse bone marrow cells showed negative results. There were no adverse influences on growth and development, biochemical indexes, organ-body ratios and histopathological changes of rats by 30 d feeding test. Conclusion Toxicity test showes that glycopeptides from A. davidianus have no obvious poisonous effects. KEY WORDS: Andrias davidianus; glycopeptides; toxicity; mice; rat 1 引言 大鲵(Andrias davidianus)属两栖纲、有尾目、隐鳃鲵科, 是我国特有的珍稀物种, 属国家二类保护动物, 主要分布于长江中上游、珠江中上游及汉水上游的溪流中[1]。20世纪90年代以来, 大鲵的人工养殖技术取得了重大突破。目前, 全国大鲵人工繁殖单位已达100余个, 年人工繁育大鲵幼苗达10余万尾, 2015年大鲵养殖总量已接近600万尾。因此, 为了提高大鲵养殖业的经济效益, 大鲵的精深加工成为十分紧迫的任务[2]。大鲵皮肤表面具有丰富的黏液腺和颗粒腺, 这些腺体能分泌大鲵黏液[3]。Qu等[4]利用Aspergillus sp.酸性蛋白酶酶解大鲵体表黏液, 获得了分子量小于3500 Da的大鲵低聚糖肽。佟长青等[5]利用冷冻浓缩工艺获得了大鲵低聚糖肽粉。曲敏等[6]利用昆明种小鼠研究了不同剂量的大鲵低聚糖肽对小鼠免疫功能的影响,