金属硫蛋白对仔猪抗氧化功能及SOD基因表达影响.docVIP

金属硫蛋白对仔猪抗氧化功能及SOD基因表达影响 　 作者：李丽立，刘云华，侯德兴，印遇龙，张彬，侯振平，邱细敏 【关键词】 金属硫蛋白；抗氧化酶；基因表达；仔猪 【Abstract】 AIM: To inverstigate the effects of exogenous Znmetallothionein (ZnMT) on antioxidative function and gene expression of SOD in weaning piglets. METHODS: Eighteen piglets (Duroc×Landrace×Yorkshire) were selected and divided into 3 groups (1, 2 and 3) randomly. The piglets were sport to produce stress. ZnMT of piglet liver dissolved in physiological saline were then injected into the piglets of group 1, 2 and 3 with the concentrations of 0, 0.8, 1.6 mg/kg, respectively. Three and 6 h later, 3 piglets were selected from each group randomly and slaughtered to get liver samples. The biochemical indexes related to antioxidation and the level of SOD gene expression in liver were determined. RESULTS: After ZnMT injection, the activities of SOD and GSHPX increased significantly (Plt;0.05)， the content of MDA decreased significantly (Plt;0.05), and the level of antireactive oxygen species and antisuperoxide anion had the trend of improvement. Six hours after ZnMT injection, the level of SOD gene expression in group 2 and 3 increased significantly as compared with that in group 1 (Plt;0.05). The level of SOD gene expression in group 2 and 3 at 6 h enhanced significantly as compared with that at 3 h. Thus, our data indicated that ZnMT treatment stimulated SOD mRNA expression in a time and dosedependent manner. CONCLUSION: Activity of antioxidase can be increased by supplement of ZnMT, thereby improving the power of antistress. 【Keywords】 metallothionein; antioxidative enzyme; gene expression; piglet 【摘要】 目的：用仔猪作模型，研究经锌元素诱导的外源性金属硫蛋白（ZnMT）对机体抗氧化功能和超氧化物歧化酶(SOD)基因表达的影响. 方法：选用杜长大杂交仔猪18头，随机分为3组(1,2和3）. 分别肌肉注射经生理盐水溶解的猪肝ZnMT 0 mg/kg (1组），0.8 mg/kg （2组）, 1.6 mg/kg （3组），让仔猪运动产生应激. 注射MT后3 h和6 h，分别从每组取3头仔猪屠宰取肝脏，测定肝脏中与抗氧化有关的生化指标，检测肝脏SOD基因表达水平. 结果：在应激条件下，补充外源性ZnMT一段时间后，仔猪肝脏SOD，GSHPX活性显著升高(Plt;0.05)，MDA含量显著降低(Plt;0.05)，肝脏抗活性氧和抗超氧阴离子水平也有提高的趋势. 在注射ZnMT后6 h，0.8 mg/kg，1.6 mg/kg组仔猪肝脏SOD基因表达水平比对照组显著提高(Plt