镁对高脂高糖膳食诱导大鼠肥胖影响及其机理探析.docVIP

镁对高脂高糖膳食诱导大鼠肥胖影响及其机理探析 　中国论文联盟编辑。 作者：彭晓莉　吕晓华　李茂全 毕业论文 【摘要】 　探讨补镁对高脂高糖膳食诱导大鼠肥胖模型形成的 影响 及作用机制。 方法 以雄性SD大鼠为实验对象，用高脂高糖诱导大鼠肥胖模型。将大鼠按体重随机分为6组：A组为普通饲料组、B组为低镁饲料组、C组为高脂高糖饲料组、D组为低剂量补镁组、E组为中剂量补镁组，F组为高剂量补镁组。第9周末处死动物，腹主动脉取血测血糖、血清TC、TG、HDL、VLDL，放射免疫法测血清胰岛素，免疫组织化学方法测定大鼠脂肪组织和骨骼肌肿瘤坏死因子-α(TNF-α)和骨骼肌一氧化氮合酶(NOS)表达。结果 膳食中补充镁的大鼠体重、脂体比、脂肪细胞面积低于高脂高糖饲料组（P＜0.05），HDL、TC、TG、血糖和胰岛素抵抗指数(HOMA-IR)与高脂高糖饲料组大鼠相比出现降低（P＜0.05），VLDL升高（P＜0.05），补镁大鼠骨骼肌TNF-α的表达，脂肪组织和骨骼肌NOS的表达降低（P＜0.05）。结论 在高脂高糖饲料诱导大鼠肥胖的同时补镁能有效预防肥胖的发生，改善脂代谢和胰岛素抵抗状态。镁可能通过对TNF-α和NOS表达的调节发挥作用。 论文代写 【关键词】 镁；肥胖；胰岛素抵抗；肿瘤坏死因子-α；一氧化氮合酶 Abstract：To explore the effect of Mg on the obesity in rats fed with high fat and sucrose diet.Methods SD rats were randomly divided into six groups and respectively fed with different diets.Obese rat model was induced by high fat and sucrose diet.At the end of experiment,the rats were sacrificed. The blood glucose,serum TC,TG,HDL,VLDL were measured respectively.The level of TNF-α and NOS were measured with immuno-chemistry technique.Results the body wEight,the body-fat ratio,the adipocyte areas of Mg supplementation groups were lower significantly than group C(P＜0.05).HDL,TC,TG,VLDL,the blood glucose,HOMA-IR of Mg supplementation groups were lower,VLDL of Mg supplementation groups was higher significantly than group C(P＜0.05).TNF-α expression in skeletal muscle of Mg supplementation groups was decreased significantly than group C(P＜0.05).NOS expression in skeletal muscle and fat tissue of Mg supplementation groups was decreased significantly than group C(P＜0.05).Conclusion Mg can control the development of obesity,meliorate metabolism and insulin resistance,probably through regulating the level of TNF-α and the activity of NOS. Key words:magnesium;obesity;insulin resistance;tumor necrosis factor-α;nitric oxide synthase 肥胖者需要比体重正常者更多的与物质能量代谢密切相关的矿物质和维生素，以缓解机体过高的能量代谢负荷，纠正机体糖、脂类及激素代谢紊乱，维持正常的物质代谢和能量代谢［1］。镁（Magnesium，Mg）是人体必需的常量元素之一，催化和激活体内300多种酶系，参与体内所有能量代谢过程，在能量的运输、贮存及利用过程中发挥重要作用。高脂血症、高血压、糖尿病、肥