阿霉素对人胆管癌细胞株FRH0201生物学特性影响.docVIP

阿霉素对人胆管癌细胞株FRH0201生物学特性影响 　【关键词】 胆道肿瘤#12539;阿霉素#12539;肿瘤细胞,培养液#12539;细胞周期#12539;细胞大小#12539;CA125#12539;CA19 论文代写 【摘要】目的：观察阿霉素长时间作用对胆管癌细胞株FRH0201生物学活性的 影响 。 方法 ：用2μg/ml的阿霉素同一浓度逐渐延长时间作用于人胆管癌细胞株FRH0201细胞，经反复作用筛选出能在含1μg/ml的阿霉素培养液中生存72h，去掉阿霉素后仍能继续稳定传代生长的细胞。无药培养1月后进行生物学行为的监测。观察细胞形态学，绘制生长曲线和流式细胞仪测定细胞周期，酶联免疫吸附法测定细胞的肿瘤标记物，激光共聚焦显微镜观察细胞内的阿霉素的荧光强度。结果：阿霉素作用后FRH0201细胞形态学有轻度改变，细胞倍增时间较亲本细胞延长3h，与亲本细胞相比S期细胞增多16.89%，G1期细胞减少29.33%、G2期细胞增多12.46%。CA125和CA199试验组和亲本组分别为9.14和8.60U/ml，1.59和2.96U/ml，细胞内阿霉素浓度（荧光强度平均值），亲本细胞为1764.8，试验组细胞为305.4。结论：阿霉素对胆管癌细胞株FRH0201的形态学及倍增时间无明显影响，但使细胞进入S期、G2期的增多，细胞内药物浓度明显降低，生长时间无明显变化。 【关键词】胆道肿瘤#12539;阿霉素#12539;肿瘤细胞，培养液#12539;细胞周期#12539;细胞大小#12539;CA125#12539;CA199 The effects of adramycin on the human liver hilar cholangiocarcinona cell line FRH0201 【ABSTRACT】Objective:To investigate the effects of adramycin on cell line of FRH0201.Methods:Human hilar cholangiocarcinona cell line were cultured with adramycin (2μg/ml) through lasting different times,finally this cell line can survive after 72h cultured in the culture liquid with the adramycin(1μg/ml) .Then the medium was replaced with a fresh one without adramycin,and the cells were continuously cultured for 1 month.The in vitro studies included the observation of the appearance with optical microscope,MTT cell proliferation assay,analysis of the cell cycle by Flow cytometry(FACS),assays of tumor marker using enzymelinked immunosor bent assay(ELISA),the fluorescence density of adramycin.Results:Compared to the parent FRH0201cell line:The morphology showed typical morphological characteristics,the doubling time prolonged about 3h,the cell cycle by flow cytometry identified in G1,G2 and S phase of cell cycle were 40.50%,19.74%and 39.77% in trial group,and 69.83%,7.29%and 22.88% in parent group,respectively.Tumor marker has no difference between them,the fluorescence density of epirubicin descending 5.2 times.Conclusion:The adramycin can affect the cell cycle and cause the change of metabolism. 【K