麦冬指纹图谱探究.docVIP

麦冬指纹图谱探究 　 作者：陈有根　邬国庆　戴俊东　李慧 【摘要】 ：目的 建立中药麦冬的指纹图谱,为麦冬药材及其制剂的质量控制提供依据。方法 采用HPLC梯度洗脱、紫外检测的方法,分别对产于四川绵阳的川麦冬和浙江慈溪的杭麦冬各10批药材进行检测,用国家药典委员会颁布的“中药色谱指纹图谱相似度评价系统A版”,计算各药材样品的相似度,并生成麦冬药材对照指纹图谱。结果 来源于两个不同产区的药材,产区内样品间相似度多数大于0.95,但产区间样品相互比较,则相似度多数小于0.80,有的竟小于0.50。结论 川麦冬和杭麦冬的指纹图谱相差明显,不仅可用于区别两个不同产区的药材,也可用于区别用两个不同产区的药材生产的中成药制剂。 【关键词】 川麦冬　杭麦冬　指纹图谱　高效液相色谱法 　　Abstract：Objective To establish the fingerprints for characterization of the chemical components of maidong (root of Ophiopogon japonicus) in two main cultivate regions of China, Sichuan (Chuanmaidong) and Zhejiang (Hangmaidong). Methods An HPLC-UV analytical methods was applied to detect 70% ethanol extracts of 20 samples from Sichuan and Zhejiang province, a “Fingerprint similarity evaluating system for TCM” issued by Pharmacopoeia Committee of P. R. China was used to evaluate the similarities all of the samples. Results The fingerprints revealed that the similarities were higher than 0.95 between samples from the same cultivate region, and were lower than 0.80 between samples from different regions of above two. Conclusion The fingerprints of Chuanmaidong and Hangmaidong were provided with high difference, and the difference can be taken as a most important proof for distinguishing the material medica of Chuanmaidong and Hangmaidong, but also in patent medicine of TCM. 　　Key words：Chuangmaidong；Hangmaidong；fingerprints；HPLC 　 麦冬来源于百合科(Liliaceae)沿阶草属植物麦冬Ophiopogon japonicus (Thunb.) Ker-Gawl.的干燥块根,具有滋阴生津、润肺清心等功效,主要成分为甾体皂苷类、高异黄酮类和多糖类化合物。我国麦冬道地产区有两个,分别为四川绵阳和浙江慈溪,来源于两个产区的麦冬药材分别习称川麦冬和杭麦冬。由于两个产区的气候环境和采收习惯不同,导致其化学成分和含量存在一定差异,如川麦冬主含麦冬皂苷D,并含少量麦冬皂苷B,而杭麦冬主含麦冬皂苷B,含少量或不含麦冬皂苷D；川麦冬中甲基麦冬黄烷酮A的含量高于甲基麦冬黄烷酮B,而杭麦冬中甲基麦冬黄烷酮A的含量则低于甲基麦冬黄烷酮B,而且杭麦冬中甲基麦冬黄烷酮A和甲基麦冬黄烷酮B的总量显著高于川麦冬。基于比较川麦冬与杭麦冬的化学成分特征,为麦冬药材及其制剂的质量控制提供依据,本试验通过系统研究,分别建立了川麦冬、杭麦冬的指纹图谱。 　　1 仪器与试药 　 Agilent 1100高效液相色谱仪,四元泵,自动进样器,柱温箱,DAD二极管阵列检测器,Agilent A10.02工作站。Kromasil C18色谱柱(4.6 mm×250 mm,5 μm)。样品来源与编号见表1。乙腈为色谱纯,水为纯净水,其它试剂均为分析纯。 表1 药材来源（略） 　　2 方法与结果 　　2.1 提取方法的选择 　 我们对《中华