麻红止哮合剂对哮喘气道重塑大鼠血管内皮生长因子及碱性成纤维细.docVIP

麻红止哮合剂对哮喘气道重塑大鼠血管内皮生长因子及碱性成纤维细 　【摘要】 目的 观察麻红止哮合剂对与哮喘气道重塑密切相关的血管内皮生长因子(VEGF)与碱性成纤维细胞生长因子(b-FGF)的影响,探讨其治疗哮喘慢性持续期的作用途径。方法 SD大鼠40只,随机分为正常组、造模组、激素组和中药组。以腹腔注射100 g/L卵蛋白(OVA) 1 mL和10 g/L 的OVA吸入激发制成支气管哮喘模型。激素组和中药组在每次雾化前20 min给予强的松或中药干预。空白组和造模组在每次雾化前20 min给予2 mL生理盐水。用ELISA法测定血清VEGF与b-FGF浓度。结果 造模组血清VEGF与b-FGF浓度较正常组、激素组和中药组明显增加(Plt;0.05)。正常组、激素组和中药组组间比较,血清VEGF浓度有显著差异(Plt;0.05)；正常组、激素组和中药组组间比较,血清b-FGF浓度无显著性差异(Pgt;0.05)。结论 麻红止哮合剂治疗哮喘慢性持续期的作用途径,可能有一部分是通过降低血清VEGF、b-FGF浓度来延缓支气管哮喘气道重塑的形成,具有和强的松相似的效果。 【关键词】 支气管哮喘；气道重塑；血管内皮生长因子；碱性成纤维细胞生长因子；大鼠 Abstract：Objective To investigate the effect of Mahong decoction on serum VEGF and b-FGF of rat model bronchus asthma airway remolding. Method Forty SD rats randomly divided into four groups： blank group, asthma group, hormone group, Chinese medicine group. The asthmatic model was established by sensitization and challenge with ovalbumin, and the rats in the hormone group and Chinese medicine group conferred hormone and Chinese medicine before the challenge. ELISA was used to measure the level of serum VEGF and b-FGF. Results The serum VEGF and b-FGF in asthma group was higher than in other groups (Plt;0.05). The level of serum VEGF in blank group, hormone group, Chinese medicine group were different (Plt;0.05). The level of serum b-FGF in blank group, hormone group, Chinese medicine group were similar (Pgt;0.05). Conclusion Mahong decoction may control the level of serum VEGF and b-FGF of rat model of bronchus asthma airway remolding, which has the similar result with hormone. 　　Key words：bronchus asthma；airway remolding；VEGF；b-FGF；rat 麻红止哮合剂是本院呼吸科治疗支气管哮喘的常用方剂,在临床上取得了较好的疗效。我们建立了大鼠支气管哮喘模型(以下简称哮喘模型),以观察其气道重塑的改变,通过麻红止哮合剂对其干预,初步探讨其治疗哮喘慢性持续期的作用途径,观察其对与哮喘气道重塑密切相关的血清血管内皮生长因子(vascular endothelial growth factor,VEGF)与碱性成纤维细胞生长因子(basic fibroblast growth factor,b-FGF)的影响,为临床应用提供可靠的理论依据。 　　1 实验材料 　　1.1 动物 　　健康雄性SD大鼠40只,体重(200±10)g,北京维通利华实验动物技术有限公司提供[许可证号：SCXK(京)2002－0003]。 　　1.2 药物 　　卵蛋白(ovalbumin,OVA)GradeⅡ,购于Sigma公司。灭活百日咳杆菌苗