MAGE-3 在胃癌组织中表达及其临床意义.doc

MAGE-3 在胃癌组织中表达及其临床意义 　 作者：何宋兵　汪良　吴雨岗　陈彦　孙康　张雁云 【摘要】 　　目的: 检测黑色素瘤抗原基因-3 (MAGE-3) 在胃癌组织中的表达，探讨其在胃癌免疫治疗中的应用价值。方法: 应用 RT-PCR方法对 40 例胃癌组织和相应的癌旁组织以及 20 例胃慢性炎症组织中的 MAGE-3 基因进行检测，随机选取 RT-PCR 阳性扩增产物进行测序，并结合临床病理参数进行研究分析。结果: 在 40 例胃癌组织中，MAGE-3 基因的表达阳性率为 67.5% (27/40)，明显高于在相应的癌旁组织 15% (6/40)及胃慢性炎症组织中的表达阳性率 10 % (2/20)。DNA 测序证实 RT-PCR 产物确为 MAGE-3 基因的目的片段。胃癌组织中 MAGE-3 基因表达的阳性率与胃癌的浸润深度有关，而与病理分期、分化程度和淋巴转移无 关。结论: MAGE-3 基因在胃癌组织中有较高的表达率，可望将其编码的蛋白作为靶分子应用于胃癌的免疫治疗。 【关键词】 胃肿瘤· 黑色素瘤抗原基因-3 　　Expression of melanoma antigen gene-3(MAGE-3) in gastric neoplasm and its clinical significance 【ABSTRACT】 Objectives: To detect the expression of melanoma antigen-3(MAGE-3) in human stomach neoplasms and explore the possibility of applying the antigen as target for immunotherapy of stomach neoplasms. Methods: Expression of MAGE-3 gene in 40 stomach neoplasms, para-tumorous mucosa, and 20 samples of chronic gastritis were examined by reverse transcription-polymerase chain reaction (RT-PCR). The samples selected randomly from each gene with positive PCR were confirmed by DNA sequence analysis. Results: In 40 stomach neoplasms, MAGE-3 gene was expressed in 27 tumor tissues (67.5%). The positive rates of MAGE-3 gene expression in neoplasm tissues were significant higher than those of para-tumorous mucosa (15%) and 20 samples of chronic gastritis (10%). The DNA sequence confirmed that the RT-PCR products was truly target cDNA. The expression of MAGE-3 gene in stomach neoplasm tissues was relevant to the intruded depth of neoplasm cells (P＜0.05), but not relevant to pathological staging, the differentiatation degree of neoplasm cells and lymph node metastasis (P＞0.05). Conclusion: MAGE-3 gene is expressed in stomach neoplasm at a high frequency, which suggests the possibility of using them as targets for antigen-specific immunotherapy. 【KEY WORDS】 Stomach neoplasms · Melanoma antigen gene-3 · Gene expression 　　胃癌是常见的恶性肿瘤之一，其病因复杂、异质性高、预后差，目前尚未发现其特异性较高的肿瘤相关抗原。黑色素瘤抗原基因 (Melanoma antigen gene，MAGE) 首先发现于黑色