可降解复合人工骨修复骨缺损实验探究.docVIP

可降解复合人工骨修复骨缺损实验探究 　 作者：陈建民，刘方刚，金大地 【摘要】 ［目的］探讨硫酸钙（CS）/骨基质明胶（BMG）复合人工骨修复节段性骨缺损的能力。［方法］分别制备CS、BMG，按一定比例复合，植入兔尺骨15 mm骨缺损，并随机分为3组，CS/BMG组植入复合人工骨、CS组单纯植入硫酸钙、空白对照组缺损区旷置。标本于术后4、8、12周分批取材，经大体观察、影像学、组织学及四环素荧光示踪检测，观察修复骨缺损的效果。［结果］术后切口均一期愈合，植入区周围未见炎性细胞浸润。CS/BMG组植入4周，缺损区两端及中央均可见岛状新骨生长，8周时材料已完全降解，新骨生长活跃，并逐渐由编织骨重塑为板层骨，12周缺损区被新生骨替代，骨结构逐渐成熟，与宿主骨间形成骨性桥接，髓腔再通，完成骨性修复。CS组4周两端也见新骨生长，但较CS/BMG组成骨量少，中央部位新骨出现时间晚。8周时材料完全降解吸收，植入区可见大量骨痂生长，两端出现板层样新骨，12周缺损区得到基本修复，但骨髓腔轮廓不清。空白组术后形成骨不连。［结论］。CS/BMG复合人工骨具有良好的生物相容性和可降解性，能有效地修复兔尺骨骨缺损，是一种较为理想的骨移植替代材料。 【关键词】 硫酸钙；骨基质明胶；人工骨；骨缺损；骨移植 Abstract：［Objective］To investigate the osteogenetic effect of calcium sulfate（CS）combined with bone matrix gelatin（CS）as bone substitute material in a rabbit ulnar defect model.［Method］Twenty one adult New Zealand White rabbits were used.The segmental ulnar defects of 15 mm were created b/laterally and were divided into tbree groups:Group 1,defects were filled with CS/BMG;Group 2,defects were filled with CS;Group 3,untreated control defects.The samples were harvested at 4,8,12 weeks and were examined by radiographic,histomorphological and tetracycline tracing analysis.［Result］No inflammatory or foreign body reaction was observed around all the defects.Both CS/BMG and CS were almost completely resorbed 8 weeks postoperation.At the group 1,nem bone was found throngbout the defects after 4 weeks.All defects were repaired with a wellorganized traheeular pattern and a thin neocortex after 12 weeks.At the group 2,mass of new bone tissue formed at the periphery of the defect 4 weeks postoperatlon,but the development of new bone at the central of the defect was latter than that at the group 1.At 12 weeks,all defects were bridged by new bone tissue with little adjacent medullary bone.At the group 3,slight new bone was observed at the periphery of the defect region,and the defects were filled with fibrous tissue.［Conclusion］Calcium sulfate containing bone matrix gelatin is totally bioresorbable and biocompatible.The compound material c