四黄汤对实验性糖尿病大鼠肾脏保护作用探究.docVIP

四黄汤对实验性糖尿病大鼠肾脏保护作用探究 　 作者：李占林 高永荣 王惠锋 陈桂敏 薄爱华 【摘要】 目的：探讨四黄汤对实验性糖尿病（DM）大鼠肾脏保护作用的机理。方法：Wistar大鼠40只，链脲佐菌素（STZ）注射方法造模，将造模成功的32只大鼠随机分为模型组、四黄汤大剂量组、四黄汤小剂量组、开博通组4组，每组8只。另选8只性别、体重配对的大鼠做正常对照组。大剂量组给予四黄汤液40g/kg/d，小剂量组给予四黄汤液20g/kg/d，开博通组给予开博通混悬液10mg/kg/d，以上均每日一次，总疗程6周。实验过程中记录动物一般状态，并在最后一次给药后，禁食8h，称重后断头取血检测血糖、胰岛素、血脂、尿微量白蛋白、内皮素、血栓素等各项指标。取左肾称重，右肾固定，按常规制作石蜡切片和超薄切片，在光镜和电镜下观察肾脏病理变化。结果：四黄汤大剂量组的血糖、血脂、尿微量白蛋白、胰岛素、内皮素、血栓素各项指标均有明显改善。结论：四黄汤对实验性糖尿病（DM）大鼠肾脏有保护作用。 【关键词】 糖尿病;四黄汤;开博通;大鼠;肾脏 【ABSTRACT】 Objective:To study the protective effects of sihuangtang on the diabetic nephropathy and its mechanisims.Methods:Forty adult male wistar rats were studied,the diabetes model was established by intraperitoneal injection of STZ. Then 32 wistar rats were divided into 4 groups randomly: the model group,the highdose sihuangtang group, the low-dose sihuangtang group and the captopril group, 8 rats for each group. Another 8 rats with similar weight and same sex were choser to work as control group.For highdose group,40g/kg/d was injected while 20g/kg/d for lowdose group and 10mg/kg/d for cabtopril group. The mocess would last bweeks.The records of the average situation would be kept during the process.After final admimistration the rats would be fasted for 8h. Then weigh them and cut the heads to examine the indexs of the plasma glucose, insulin, blood faturine trace, albumin, ET and TXB2. Take out the left kidney and fix the right to make the paraffin and thinest slices. At last the variations of the pathology were observed under electrical microscope.Results: The Indexes of plasma glucose、 insulin、 blood faturine trace, albumin, ET and TXB2 were decreased remarkably.Conclusion: sihuangtang can protect the kidney of rats with diabetes. 【KEY WORDS】 Diabetic mellitus;Sihuangtang;Cabtopril;Rat;Kideny 糖尿病肾病（diabetes nephropathy DN）是糖尿病（diabetes mellitus DM）常见的严重微血管并发症之一，是糖尿病患者过早死亡及生存质量下降的主要原因，其发病率约占糖尿病患者的35%～40%[1]。西药治疗糖尿病在降糖方面疗效肯定，但大多具有不同程度的不良反应，同时也不能有效控制其并发症，而中医药对于糖尿病并发症的防治显示了其明显的优势。因此发挥中医药优势，探索理想药物具有