小脑移植基础及临床探究.docVIP

小脑移植基础及临床探究 　　　【摘要】　目的　为了证实小脑组织移植后移植物在受体脑内是否存活以及植入的神经细胞是否可以纠正和改善小脑功能缺陷和症状。方法　将胎龄为13天的C57BL/6小鼠胚胎小脑组织悬液移植到3个月龄的BALB/c成年小鼠脑内，术后观察1～8周，并进行组织学观察。在动物实验基础上，将胎龄为9～12周的人胚胎小脑组织悬液移植到6例患有严重小脑萎缩的患者脑内。结果　动物实验的组织切片证实移植物在受体脑内再生，并且可见浦氏细胞分化成熟和迁徙到颗粒细胞层。6例患者接受小脑移植后近期疗效良好，症状和体征及客观指标均有改善。经过5～7年随访，仅2例患者疗效稳定。结论　未成熟小脑组织移植后可在受体脑内存活，并且有神经细胞再生和改善神经功能潜力，但术后的排斥反应尚需深入研究。 关键字： 小脑/移植 移植物生存 　　 　　【Abstract】　Objective　To verify whether the cerebellar cell grafts to be survival in the recipient brain and the transplanted neurons to correct and improve the cerebellar function disorders and symptoms so as to explore a new way to treat cerebellar lesions.Methods　Embryonic cerebellar tissue suspension from 13-day C57BL/6 mice was transplanted into the injured cerebellum of 3-month BALB/c mice. After operation, observation(including histological observation)was conducted for 1 to 8 weeks. On the basis of animal experiment, 9～12-week human embryonic cerebellar tissue suspension was transplanted into the cerebellum of 6 medically intractable patients with severe hereditary cerebellar degenerative ataxia.Results　The histological observation of the cerebellar tissue in the animal experiment revealed that embryonic grafts could survive and that mitoses of the host Purkinje cells of the implanted group were increased substantially over the control groups. The short-term therapeutic effects were good in the 6 patients following cerebellar transplantation. The symptoms, signs, and objective parameters were all improved to some extent. After 5 to 7 years’ follow-up, only 2 patients had a good result.Conclusion　The embryonic cerebellar tissue can survive in the recipient cerebellum and has the potential to regenerate the neurons and improve the nervous function. 　　【Key words】　Cerebellum/transplantation　　Graft survival 　　自1987年4月以来，我院与美国犹他州立大学医学中心合作，进行了胚胎小脑组织移植的动物实验研究。在此基础上，我们对6例重度小脑萎缩的患者进行了胚胎小脑组织脑内移植，现将实验资料和临床资料报告如下。 　　实验资料 医学 　　供体为胎龄13天的C57BL/6小鼠，其细胞采用单克隆抗体蛋白标记。受体选用3个月龄的BALB/c小鼠56只，该鼠的神经细胞不为上述单克隆抗体蛋白所染色。 　　将BALB/c