高强度聚焦超声辐照离体组织中空化泡与沸腾泡对辐照后即刻.docVIP

高强度聚焦超声辐照离体组织B超声像图中强回声的 艾慧坚，钟明松，李发琪*，王 彬，易华容，王 琦，王智彪（）摘要研究高强度聚焦超声（igh intensity focused ultrasound，HIFU）辐照离体牛肝组织中的空化泡和沸腾泡对辐照后即刻B超声像图中强回声的贡献。方法 牛肝组织被随机分成组，每组20块。一组使用200 W声功率的HIFU辐照2 s，以在HIFU焦域处只产生空化泡，另一组使用50 W声功率的HIFU辐照80 s，以在焦域处只产生沸腾泡。在辐照过程中，使用置于HIFU声焦点旁1 mm的热电偶对焦域处的温度进行测量，并通过计算机的RS 232串口进行记录。同时，使用被动空化检测（passive cavitation detection，PCD）系统测取牛肝组织内传播出的声信号，并在LabVIEW开发平台上编程以获得该信号的频谱，计算该频谱在37 MHz内滤除谐波后的均方根值（Root Mean Square，RMS）。记录HIFU辐照后即刻的B超声像图，与辐照前同一位置的声像图进行比较。结果 200 W声功率的HIFU辐照离体牛肝组织后即刻B超声像图中有19次未出现回声，PCD系统所检测信号的频谱中出现明显的宽带噪声，辐照开始后RMS增大，焦点处的最高温度为61.10 ± 6.18）℃。50 W声功率的HIFU辐照离体牛肝组织后即刻B超声像图中有19次出现强回声，PCD系统所测信号频谱中未见次谐波和宽带噪声，RMS未见增大，焦域处的最高温度为93.34 ± 3.37)℃。辐照后，所有牛肝组织中均出现了凝固性坏死。结论 HIFU辐照后即刻B超声像图中强回声出现的原因是辐照过程中产生的沸腾泡，非空化泡。 [关键词] 高强度聚焦超声；B超声像图；强回声；空化泡；沸腾泡 中图分类号：R454.3 Effect of Cavitation Bubbles and Boiling Bubbles to Hyperecho on B-mode Ultrasound Image after Ex Vivo Tissue Exposed to High Intensity Focused Ultrasound (HIFU) Ai Huijian, Zhong Mingsong, Li Faqi*, Wang Bin, Yi Huarong, Wang Qi, Wang Zhibiao （Chongqing Municipal Key Laboratory of Ultrasound Engineering in Medicine, State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and MOST, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016；National Engineering Research Center of Ultrasound Medicine, Chongqing, 401121 China） [Abstract] Objective To investigate the effect of cavitation bubbles and boiling bubbles to hyperecho on B-mode ultrasound image after ex vivo bovine liver tissue exposed to high intensity focused ultrasound (HIFU) respectively. Methods 20 ex vivo bovine livers were exposed to HIFU with 200 W acoustic power for 2 s so there were only cavitation bubbles at the focal region of HIFU, other 20 bovine livers were exposed to HIFU with 50 W acoustic power for 80 s to get only boiling bubbles at the focal region. During these exposures, the temperatures at the focal region of HIFU were measured by a thermocouple embedded into livers next to the