姜黄素的抗氧化及抗肿瘤活性研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.12 姜黄素的抗氧化及抗肿瘤活性研究 陈建平，李琳，苏健裕 （华南理工大学轻工与食品学院，广东广州 510640） 摘要：本文采用分光光度法测定姜黄素对ABTS 和DPPH 自由基的清除能力；运用AAPH 诱导红细胞氧化性溶血考察姜黄素对 AAPH 诱导人血红细胞损伤的抑制作用。通过MTT 方法考察姜黄素对A375 恶性黑色素瘤生长状态的影响，并用流式细胞仪检测细 胞凋亡数量；运用Western blot 测定姜黄素对JNK 和Akt 蛋白表达的影响。结果表明，姜黄素对DPPH 和ABTS 自由基具有较好的清 除能力，呈浓度和时间依赖性；同时，姜黄素能有效抑制AAPH 诱导红细胞溶血，当姜黄素为40 μM 时，溶血抑制率达到52.78±1.03% 。 MTT 结果表明，随着姜黄素浓度的升高，A375 细胞存活率逐渐下降，当姜黄素为40 μM，A375 的细胞存活率仅为21.50±1.60%。流 式分析发现，随着姜黄素浓度的提高，细胞凋亡峰（SubG1）的含量逐渐增加。当姜黄素为40 μM 时，细胞内SubG1 峰的含量达到 了63.30% 。进一步Western blot 分析发现姜黄素诱导A375 细胞凋亡与上调JNK 磷酸化的水平和下调AKt 磷酸化的水平有关。 关键词：姜黄素；抗氧化；抗肿瘤 文章篇号：1673-9078(2014)12-11-15 DOI: 10.13982/j.mfst.1673-9078.2014.12.003 Antioxidant and Antitumor Activities of Curcumin CHEN Jian-ping, LI Lin, SU Jian-yu (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: In this paper, spectrophotometric method was employed to determine the ability of curcumin to scavenge 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and the oxidative hemolysis of erythrocytes induced by 2, 2′-azobis (2-amidinopropane) dihydrochloride (AAPH) was used to investigate the inhibitory effect of curcumin on AAPH-induced damage to human erythrocytes. The effect of curcumin on the viability of A375 malignant melanoma cells was measured by MTT assay, and the population of the apoptotic cells was detected by a flow cytometer. The effect of curcumin on the expression levels phosphorylated JNK and Akt was analyzed via Western blot assay. These