the ppm operon is essential for acylation and glycosylation of lipoproteins in corynebacterium glutamicumppm操纵子是必不可少的酰化和糖基化的脂蛋白棒状杆菌glutamicum.pdfVIP

The ppm Operon Is Essential for Acylation and Glycosylation of Lipoproteins in Corynebacterium glutamicum 1,2. 3. 4 3 1,2 Niloofar Mohiman , Manuela Argentini , Sarah M. Batt , David Cornu , Muriel Masi , 5 4 1,2 Lothar Eggeling , Gurdyal Besra , Nicolas Bayan * ´ ´ 1 Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris-Sud, Orsay, France, 2 Centre National de la Recherche Scientifique UMR 8619, Orsay, France, 3 Centre National de la Recherche Scientifique, Institut de Chimie des Substances Naturelles, Gif sur Yvette, France, 4 School of Biosciences, University of ¨ Birmingham, Birmingham, United Kingdom, 5 Forschungszentrum Juelich GmbH, Julich, Germany Abstract Background: Due to their contribution to bacterial virulence, lipoproteins and members of the lipoprotein biogenesis pathway represent potent drug targets. Following translocation across the inner membrane, lipoprotein precursors are acylated by lipoprotein diacylglycerol transferase (Lgt), cleaved off their signal peptides by lipoprotein signal peptidase (Lsp) and, in Gram-negative bacteria, further triacylated by lipoprotein N-acyl transferase (Lnt). The existence of an active apolipoprotein N-acyltransferase (Ms-Ppm2) involved in the N-acylation of LppX was recently reported in M. smegmatis. Ms- Ppm2 is part of the ppm operon in which Ppm1, a polyprenol-monophosphomannose synthase, has