the pseudokinase nipi-4 is a novel regulator of antimicrobial peptide gene expression的pseudokinase nipi-4抗菌肽基因表达的是一种新型的监管机构.pdfVIP

The Pseudokinase NIPI-4 Is a Novel Regulator of Antimicrobial Peptide Gene Expression 1,2,3 1,2,3 4 1,2,3 1,2,3 Sid ahmed Labed , Shizue Omi , Martha Gut , Jonathan J. Ewbank , Nathalie Pujol * ´ ´ 1 Centre d’Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, Marseille, France, 2 Institut National de la Sante et de la Recherche Medicale (INSERM), ` ` U1104, Marseille, France, 3 Centre National de la Recherche Scientifique (CNRS), UMR7280, Marseille, France, 4 Centre Nacional d’Analisi Genomica, Barcelona, Spain Abstract Hosts have developed diverse mechanisms to counter the pathogens they face in their natural environment. Throughout the plant and animal kingdoms, the up-regulation of antimicrobial peptides is a common response to infection. In C. elegans, infection with the natural pathogen Drechmeria coniospora leads to rapid induction of antimicrobial peptide gene expression in the epidermis. Through a large genetic screen we have isolated many new mutants that are incapable of upregulating the antimicrobial peptide nlp-29 in response to infection (i.e. with a Nipi or ‘no induction of peptide after infection’ phenotype). More than half of the newly isolated Nipi mutants do not correspond to genes previously associated with the regulation of antimicrobial peptides. One of these, nipi-4, encodes a member of a nematode-specific k