the relationship between cd4+cd25+cd127- regulatory t cells and inflammatory response and outcome during shock statescd4 + cd25 + cd127的关系u2014u2014调节性t细胞和炎症反应和结果在休克状态.pdfVIP

Hein et al. Critical Care 2010, 14:R19 /content/14/1/R19 RESEARCH Open Access + + - The relationship between CD4 CD25 CD127 regulatory T cells and inflammatory response and outcome during shock states 1 2 1 1 3 François Hein , Frédéric Massin , Aurélie Cravoisy-Popovic , Damien Barraud , Bruno Levy , Pierre-Edouard Bollaert1, Sébastien Gibot1,3* Abstract Introduction: Although regulatory T lymphocytes (Tregs) have a pivotal role in preventing autoimmune diseases and limiting chronic inflammatory conditions, they may also block beneficial immune responses by preventing sterilizing immunity to certain pathogens. Methods: To determine whether naturally occurring Treg cells have a role in inflammatory response and outcome during shock state we conducted an observational study in two adult ICUs from a university hospital. Within 12 hours of admission, peripheral whole blood was collected for the measurement of cytokines and determination of lymphocyte count. Sampling was repeated at day three, five and seven. Furthermore, an experimental septic shock was induced in adult Balb/c mice through caecal ligation and puncture. Results: Forty-three patients suffering from shock (26 septic, 17 non septic), and 7 healthy volunteers were included. The percentage of Tregs increased as early as 3 days after the onset of shock, while their absolute number remained lower than in healthy volunteers. A similar pattern of Tregs kinetics was found in infected and non infected patients. Though there was an inverse correlation between severity scores and Tregs percentage, the time course of Tregs was similar between surv