the relationship between fenestrations, sieve plates and rafts in liver sinusoidal endothelial cells之间的关系来袭,筛板和木筏在肝脏正弦内皮细胞.pdfVIP

The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells 1. 1. 2 3 4 Dmitri Svistounov , Alessandra Warren , Gregory P. McNerney , Dylan M. Owen , Dusan Zencak , 1 1 2 4 ˚ 5 Svetlana N. Zykova , Harry Crane , Thomas Huser , Ronald J. Quinn , Bard Smedsrød , David G. Le Couteur1*, Victoria C. Cogger1 1 Centre for Education and Research on Ageing and ANZAC Medical Research Institute, University of Sydney and Concord Hospital, Sydney, Australia, 2 NSF Center for Biophotonics Science and Technology, University of California Davis, Sacramento, California, United States of America, 3 Centre for Vascular Research, University of New South Wales, Sydney, Australia, 4 Eskitis Institute, Griffith University, Brisbane, Australia, 5 Department of Medical Biology, University of Tromso, Tromso, Norway Abstract Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was an inverse distribution between sieve plates and membrane rafts visualized by structured illum