the role of natural killer (nk) cells and nk cell receptor polymorphisms in the assessment of hiv-1 neutralization的角色自然杀伤(nk)细胞和nk细胞受体多态性在hiv - 1中和的评估.pdfVIP

The Role of Natural Killer (NK) Cells and NK Cell Receptor Polymorphisms in the Assessment of HIV-1 Neutralization Bruce K. Brown1,2, Lindsay Wieczorek1,2, Gustavo Kijak1,2, Kara Lombardi1,2, Jeffrey Currier1,2, 1,2 3 1,4 1,5 1,5 Maggie Wesberry , John C. Kappes , Viseth Ngauy , Mary Marovich , Nelson Michael , 3 6 1,5 Christina Ochsenbauer , David C. Montefiori , Victoria R. Polonis * 1 Military HIV Research Program (MHRP), Rockville, Maryland, United States of America, 2 The Henry M. Jackson Foundation, Rockville, Maryland, United States of America, 3 University of Alabama at Birmingham, Birmingham, Alabama, United States of America, 4 Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand, 5 Walter Reed Army Institute of Research, Rockville, Maryland, United States of America, 6 Duke University, Durham, North Carolina, United States of America Abstract The importance of innate immune cells in HIV-1 pathogenesis and protection has been highlighted by the role of natural killer (NK) cells in the containment of viral replication. Use of peripheral blood mononuclear cells (PBMC) in immunologic studies provides both HIV-1 target cells (ie. CD4+ T cells), as well as anti-HIV-1 effector cells, such as NK cells. In this study, NK and other immune cell populations were analyzed in HIV-negative donor PBMC for an impact on the anti-HIV activity of polyclonal and monoclonal antibodies. NK cell percentages were significantly higher in donor PBMC that supported lower levels of viral replication. While the percentage of NK cells was not directly associated wit