the salmonella enterica phop directly activates the horizontally acquired spi-2 gene ssel and is functionally different from a s. bongori ortholog沙门氏菌血清phop直接激活水平获得spi-2 ssel基因和功能上不同于美国bongori直接同源.pdfVIP

The Salmonella enterica PhoP Directly Activates the Horizontally Acquired SPI-2 Gene sseL and Is Functionally Different from a S. bongori Ortholog 1,2 . 1,3. 2 1,3 2 Ohad Gal-Mor * , Dana Elhadad , Wanyin Deng , Galia Rahav , Brett B. Finlay 1 Infectious Diseases Research Laboratory, Sheba Medical Center Tel-Hashomer, Tel-Hashomer, Israel, 2 Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, 3 Sackler Faculty of Medicine, Tel Aviv University, Tel-Aviv, Israel Abstract To establish a successful infection within the host, a pathogen must closely regulate multiple virulence traits to ensure their accurate temporal and spatial expression. As a highly adapted intracellular pathogen, Salmonella enterica has acquired during its evolution various virulence genes via numerous lateral transfer events, including the acquisition of the Salmonella Pathogenicity Island 2 (SPI-2) and its associated effectors. Beneficial use of horizontally acquired genes requires that their expression is effectively coordinated with the already existing virulence programs and the regulatory set-up in the bacterium. As an example for such a mechanism, we show here that the ancestral PhoPQ system of Salmonella enterica is able to regulate directly the SPI-2 effector gene sseL (encoding a secreted deubiquitinase) in an SsrB-independent manner and that PhoP plays a part in a feed-forward regulatory loop, which fine-tunes the cellular level of SseL. Additionally, we demonstrate the presence of conserved cis regulatory elements in the promoter region of sseL and show direct binding of purified PhoP to this region. Interestingly, in contrast to the S.