the sars-unique domain (sud) of sars coronavirus contains two macrodomains that bind g-quadruplexessars冠状病毒的sars-unique域(sud)包含两个macrodomains绑定g-quadruplexes.pdfVIP

The SARS-Unique Domain (SUD) of SARS Coronavirus Contains Two Macrodomains That Bind G-Quadruplexes 1 2 2 2 1 1 Jinzhi Tan , Clemens Vonrhein , Oliver S. Smart , Gerard Bricogne , Michela Bollati , Yuri Kusov , 1 1 1 1,3 Guido Hansen , Jeroen R. Mesters , Christian L. Schmidt , Rolf Hilgenfeld * ¨ ¨ 1 Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lubeck, Lubeck, Germany, 2 Global Phasing Ltd., Sheraton House, Castle Park, Cambridge, United Kingdom, 3 Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, Hamburg, Germany Abstract Since the outbreak of severe acute respiratory syndrome (SARS) in 2003, the three-dimensional structures of several of the replicase/transcriptase components of SARS coronavirus (SARS-CoV), the non-structural proteins (Nsps), have been determined. However, within the large Nsp3 (1922 amino-acid residues), the structure and function of the so-called SARS- unique domain (SUD) have remained elusive. SUD occurs only in SARS-CoV and the highly related viruses found in certain bats, but is absent from all other coronaviruses. Therefore, it has been speculated that it may be involved in the extreme pathogenicity of SARS-CoV, compared to other coronaviruses, most of which cause only mild infections in humans. In order to help elucidate the function of the SUD, we have