the sno oncogene antagonizes wingless signaling during wing development in drosophilasno致癌基因对抗无翼信号在果蝇的翅膀发展.pdfVIP

The Sno Oncogene Antagonizes Wingless Signaling during Wing Development in Drosophila 1. 1. 2 3 1 Janine C. Quijano , Michael J. Stinchfield , Brad Zerlanko , Ying Y. Gibbens , Norma T. Takaesu , 2 2 1 Cathy Hyman-Walsh , David Wotton , Stuart J. Newfeld * 1 School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America, 2 Department of Biochemistry and Molecular Genetics, and Center for Cell Signaling, University of Virginia, Charlottesville, Virginia, United States of America, 3 Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America Abstract The Sno oncogene (Snoo or dSno in Drosophila) is a highly conserved protein and a well-established antagonist of Transforming Growth Factor- b signaling in overexpression assays. However, analyses of Sno mutants in flies and mice have proven enigmatic in revealing developmental roles for Sno proteins. Thus, to identify developmental roles for dSno we first reconciled conflicting data on the lethality of dSno mutations. Then we conducted analyses of wing development in dSno loss of function genotypes. These studies revealed ectopic margin bristles and ectopic campaniform sensilla in the anterior compartment of the wing blade suggesting that dSno functions to antagonize Wingless (Wg) signaling. A subsequent series of gain of function analyses yielded the opposite phenotype (loss of bristles and sensilla) and further suggested that dSno antagonizes Wg signal transduction in target cells. To date Sno family proteins have not been reported to influence the Wg pathway during development