抗中性粒细胞胞浆抗体在风湿性疾病中的作用（The role of antineutrophil cytoplasmic antibodies in rheumatic diseases）.doc

抗中性粒细胞胞浆抗体在风湿性疾病中的作用（The role of antineutrophil cytoplasmic antibodies in rheumatic diseases） The role of antineutrophil cytoplasmic antibodies in rheumatic diseases Update Date: 08-27, Zhang Wen, Ceng Xiaofeng I. The history of antineutrophil cytoplasmic antibodies (ANCA) Antineutrophil cytoplasmic antibodies are a heterogeneous group of autoantibodies targeting the cytoplasmic target antigens of neutrophils and monocytes. In 1982, Davies et al reported the presence of an anti neutrophil cytoplasmic IgG antibody in sera of 8 patients with segmental necrotizing glomerulonephritis. At that time the discovery did not pay attention, until 1985, Vander Wonde found that the antibody with wegeners granulomatosis (WG) activities are closely related, and named it the anti cytoplasmic antibody (ACPA). Since then, the antibody has attracted wide attention, and a lot of research work has been carried out, such as cloning target antigens, the mechanism of antibody causing vasculitis, and the relationship with clinical diseases. Since neutrophils are usually used as antigens to detect antibodies, they are called antineutrophil cytoplasmic antibodies (ANCA), but these antigens are in monocytes except neutrophils. In January 1988, at the first international ANCA/ACPA conference in Copenhagen, ANCA was detected by indirect immunofluorescence and ELISA. It is known that, ANCA and systemic vasculitis (such as WG), eosinophilic granuloma (CSS), microscopic polyangiitis (MPA), the lack of primary immune complexes in necrotizing glomerulonephritis are closely related, can also occur in other diseases, such as: collagen vascular disease, inflammatory bowel disease and infection the disease, 1, 2. Two, ANCA detection methods and classification Currently, three methods are used to detect ANCA: indirect immunofluorescence, ELISA and radioimmunoassay. Indirect immunofluorescence is the gold standard method of ANCA measurement of the internationally recognized, the steps are as follows: first