大鼠晶状体上皮细胞中FGF受体表达年龄变化.docVIP

大鼠晶状体上皮细胞中FGF受体表达年龄变化 　【摘要】 　　目的：探讨FGF受体在不同年龄SD大鼠晶状体上皮细胞中的表达及意义。方法：SD大鼠按1，3，6mo龄分为3组，采用晶状体前囊膜铺片方式，应用荧光免疫组化技术进行FGFR1荧光免疫组化染色，检测其蛋白水平的表达；采用剥取晶状体囊膜组织方法，应用RT-PCR技术检测FGFR1在转录水平的表达。结果：在蛋白水平，FGFR1于1mo龄组表达最强（IA = 125619±13818），3mo龄组表达明显减弱（IA =65681±5911），6mo龄组表达最弱（IA =46146±3691），组间差异显著 （P lt;0.05）；FGFR1在转录水平随着年龄增长也逐渐降低，1mo龄组为1.32±0.41，3mo龄组为1.01±0.33，6mo龄组为0.68±0.20，组间差异显著（P lt;0.05）。结论：FGFR1在大鼠晶状体上皮细胞中的表达随年龄的增长而减弱。 【关键词】 成纤维生长因子　成纤维生长因子受体　晶状体上皮细胞 　　Age-related expression of FGF receptor in rat lens epithelial cells: an in vivo study Abstract AIM: To investigate the age-related expression of fibroblast growth factor (FGF) receptor in rat lens epithelial cells. METHODS: Sprague Dawley rats were divided into three groups according to their ages: 1-, 3- and 6-month-old groups. Immunofluorescence technique was used to detect the expression of FGFR1 in rat lens epithelial cells. RT-PCR was applied to identity the expression of FGFR1 mRNA in rat lens epithelial cells. Expression of FGF receptor was analyzed in the three groups. RESULTS: FGFR1 expression at protein and mRNA levels in 1-month-old group was the highest in the three groups. The FGFR1 protein and mRNA expression in 3-months-old group were lower than that of 1-month-old group. The FGFR1 protein and mRNA expression in 6-months-old group exhibited the lowest level in the three groups. CONCLUSION: There is a significant age-related decline in FGFR1 expression at protein and transcription levels in rat lens epithelial cells. · KEYWORDS: fibroblast growth factors; fibroblast growth factor receptor; lens epithelial cells 　　0引言 成纤维细胞生长因子（fibroblast growth factors，FGFs）参与晶状体上皮细胞的增殖、移行及分化行为，调控晶状体的发育及生理功能，FGF（包括aFGF和bFGF）通过与相应受体结合，引发信息传递，从而发挥其生物学作用。有关FGF，尤其是bFGF对晶状体上皮细胞增殖与分化的影响，已有较多的研究，我们拟通过RT-PCR与荧光免疫组化手段，从转录及蛋白水平检测大鼠晶状体上皮细胞中成纤维生长因子受体（FGF receptors）的表达，以及在不同年龄表达的变化，以了解FGFs与其受体间的关系。 1材料和方法 1.1材料 健康SD大鼠来自第三军医大学野战外科研究所实验动物中心，鼠龄1~6mo，雌雄不限，动物质量符合国家《实验动物管理条例》。根据鼠龄分为3个实验组，即1mo龄组（85~100g）、3mo龄组（280~310g）和6mo龄组（330~420g）。解剖显微镜（Le