子宫内膜癌中p16基因状态对端粒酶活性影响_0.docVIP

子宫内膜癌中p16基因状态对端粒酶活性影响 　 作者：王琼书, 刘宏, 张蔚, 刘元姣 【摘要】 　　目的 探讨子宫内膜癌中p16基因状态对端粒酶活性的影响。方法 用PCR技术检测癌组织中p16基因的纯合性缺失及第一外显子异常甲基化，用原位杂交法检测端粒酶的表达。结果 36例癌组织中，9例发生p16基因缺失，12例发生甲基化，未发现基因缺失与甲基化同时存在的病例；29例内膜癌端粒酶表达阳性，阳性率为80.56%。9例基因缺失标本中均有端粒酶的阳性表达,p16甲基化与否对端粒酶的表达影响无差异，但端粒酶阳性率在p16基因失活组明显高于p16基因未失活组。结论 p16基因失活可以导致激活端粒酶端粒，p16基因失活的不同状态端粒酶激活的影响存在差异。 【关键词】 子宫内膜癌 p16基因状态 端粒酶 　　Effect of Different Type of p16 Gene Expression on The Telomerase Activity in Endometrial Carcinoma 　　Abstract：Objective To study the effect of MTS1/p16 gene deletion and 5’CpG island methylation on the expression of telomerase activity in endometrial carcinoma.Methods The p16 gene deletion and 5?CpG island methylation were determined by PCR technique, The telomerase catalytic protein subunit mRNA was assessed by in situ hibridization method.Results The p16 gene deletion and 5?CpG island methylation rates in endometrial carcinoma were 25.00%（9/36）and 33.33% (12/36) respectively. The telomerse expression rate was 80.56%(29/36).In 36 cases of endometrial carcinoma, there was 9cases with p16 gene deletion and 12 cases with 5CpG island methylation,but no case with coexistence,29cases has positive telomerse expressive.Each case with p16 gene deletion has positive telomerse expressive,whereas 5’CpG island methylation has no effect on telomerse expressive. The telomerse expression rate in p16 gene deletion group is higher than that in undeletion group, p16 gene methylation had no effect on telomerse expression .Conclusion p16 gene deactivation would activate Telomerase expression. different type of p16 gene deactivation by p16gene deletion or 5?CpG island methylation has different effect on the Telomerase expression. 　　Key words：Endometrial carcinoma; MTS1/p16 gene; Telomerase activity 　　0 引言 　　近年来的研究认为子宫内膜癌的发生与端粒酶的活化有关[1]。目前尚未见内膜癌中p16基因状态对端粒酶活性影响的报告。结合既往研究，在后续实验中，我们利用原位杂交法检测子宫内膜癌组织中端粒酶催化亚单位编码基因(catalytic protein subunit mRNA，TERT)的表达，探讨子宫内膜癌中p16基因状态对端粒酶活性的影响以及端粒酶活性表达与子宫内膜癌发生发展关系。 　　1 材料与方法 　　1.1 标本来源 检测的肿瘤标本取自1997年5月～199