基底细胞鸡尾酒标记法在前列腺穿刺组织中应用价值.docVIP

基底细胞鸡尾酒标记法在前列腺穿刺组织中应用价值 　【关键词】 前列腺肿瘤；免疫组织化学；基底细胞鸡尾酒；P63；34βE12 　　［摘要］ 目的：评价基底细胞鸡尾酒抗体P63/34βE12在前列腺穿刺组织中的应用及价值。方法：应用免疫组化Envision二步法，检测70例良性前列腺增生及28例前列腺腺癌中P63/34βE12的表达情况，并同时用P63、34βE12单标记法作为对照。结果：P63/34βE12鸡尾酒及P63、34βE12标记前列腺基底细胞的强阳性率分别为87.1%、60%和41.4%，鸡尾酒法与单标记法相比具差异有显著性，(P＜0.01)，且P63与34βE12的强阳性率二者差异具有显著性意义(P＜0.05)。结论：鸡尾酒标记法能明显提高对基底细胞免疫反应的强度和敏感性，且特异性强，可作为前列腺疾病诊断及鉴别诊断的一种有效辅助手段，具有重要的临床应用价值。 　　［关键词］ 前列腺肿瘤；免疫组织化学；基底细胞鸡尾酒；P63；34βE12 　　Value and Application of Basal Cell Cocktail(P63/34βE12)in Prostate Needle Biopsy Specimens 　　Abstract:Objective To evaluate the application and value of basal cell cocktail antibodies(P63/34βE12)in prostate needle biopsy tissue.Methods Immunohistochemistry Envision 2step techniques was used to detect the expression of P63/34βE12 in 70 cases of benign prostatic hyperplasia and 28 cases of prostatic adenocarcinoma,in contrast to P63 and 34βE12 single mark methods.Results The strong positive rates of the basal cells marked by P63/34βE12 cocktail,P63 and 34βE12 methods are respectively87.1%,60.0% and 41.4%.There is significantly difference between cocktail and single mark methods.The P values are both less than 0.01,with notable significance in the difference of strong positive rates of P63 and 34βE12(P＜0.001).Conclusion Cocktail marking can significantly raise the intensity and sensitivity of reaction to the immunoreaction of the basal cells.With its notable specificity,it can be used as an effective supplementary means in the diagnosis and discrimination and thus has significant application value. 　　Key words:Prostatic neoplasms;Immunohistochemistry;Basal cell cocktail;P63;34βE12 　　前列腺良、恶性疾病鉴别诊断是临床病理诊断中的难题之一，尤其是对病灶局限的穿刺活检标本，仅仅依靠常规HE切片极易造成误诊，常需借助免疫组化染色证实基底细胞的存在与否作为前列腺癌的诊断工具之一。我们把基底细胞特异性标记物P63和34βE12联合使用，采用鸡尾酒标记法进行染色，同步观察其表达情况，探讨P63/34βE12鸡尾酒抗体在前列腺穿刺组织中的应用价值。 　　1　材料与方法 　　1.1　材料　选取本院2000年至2005年前列腺穿刺活检标本共98例，其中前列腺良性增生70例，前列腺腺癌28例。患者年龄61岁~90岁，平均年龄70岁。所有标本经10%中性甲醛液固定，常规石蜡包埋，2 μm厚连续切片，每例3张，粘附于经APES处理的玻片上，分别用于P63、34βE12单标记和P63/34βE12鸡尾酒联合标记。P63及34βE1