基质金属蛋白酶-2在mRNA水平表达及乳腺癌转移关系及临床病理相关.docVIP

基质金属蛋白酶-2在mRNA水平表达及乳腺癌转移关系及临床病理相关 　 作者：武云飞，姜颖，肖岷，庄永志 【关键词】 乳腺癌；基质金属蛋白酶2；转移；RT-PCR ［摘要］ 目的 探讨乳腺癌MMP-2 mRNA表达量与乳腺癌转移的关系及临床意义。方法 采用荧光定量RT-PCR方法，分析32例乳腺原发癌和与其配对淋巴结转移癌的MMP-2 mRNA表达量，分析86例临床乳腺癌标本的MMP-2 mRNA表达与临床病理类型、临床分期、雌孕激素受体及Her-2表达的关系。结果 32例乳腺转移癌MMP-2 mRNA表达量低于原发癌（P＜0.05）；肿瘤≤2 cm的MMP-2 mRNA表达量高于肿瘤＞2 cm的表达量（P＜0.05）;组织分级为Ⅱ级、Ⅲ级的MMP-2 mRNA表达量低于Ⅰ级（P＜0.05）;淋巴结4个以上阳性组MMP-2 mRNA表达量低于淋巴结1～3个阴性组（P＜0.05）；MMP-2 mRNA表达量经过统计学分析与临床分期、病理类型、雌孕激素受体及Her-2表达无关。结论 MMP-2在mRNA转录水平的表达存在负反馈调节。MMP-2 mRNA转录水平的表达与乳腺癌转移有关，MMP-2在转录水平的表达在肿瘤较小时上调，随着肿瘤的增大其转录水平表达下调，随着肿瘤恶性程度的增加，其转录水平的表达量在下降，在肿瘤发生的早期表达上调，随着肿瘤的进展表达量下调。 ［关键词］ 乳腺癌；基质金属蛋白酶2；转移；RT-PCR Clinical and pathologic significance and relationship between expression of matrixmetalloproteinase 2 mRNA and breast cancer metastasis ［Abstract］ Objective To discuss the clinical and pathology significance of matrix metalloproteinase 2,MMP-2 mRNA and the relationship between MMP-2 mRNA expression and breast cancer metastasis.Methods Detect MMP-2 mRNA expression of 32 breast cancer, metastatic lymphnode and 86 clinical breast cancer samples by fluorence-quantitative RT-PCR and analyse the relativity between MMP-2 mRNA expression and clinical,pathology factors,the status of ER/PR and Her-2.Results MMP-2 mRNA expression in 32 metastatic lymph node is lower than in primary breast cancer（P＜0.05）.The mRNA expression of MMP-2 in tumor 2 cm is lower than in tumor≤2 cm（P＜0.05）.The MMP-2 mRNA expression in stage Ⅱ,Ⅲ is lower than in stage Ⅰ（P＜0.05）.The mRNA expression of MMP-2 in more than 4 lymphnodes positive is lower than in lymphnode 1-3 positive （P＜0.05）.The MMP-2 mRNA expression is irrelated with tissue grade,pathologic type,ER,PR and Her-2.Conclusion MMP-2 mRNA expression is upregulated in early clinical stage,lymph node metastatic early stage,and down regulated with tumor progressing.MMP-2 mRNA expression is related with the metastasis of breast cancer. ［Key words］ breast cancer;matrix metalloproteinase 2;metastasis;RT-PCR 肿瘤的转移是一个极其复杂的过程，这一过程包括肿瘤细胞从原发灶脱离