正常兔双眼视网膜电图对比及视网膜缺血-再灌注模型建立.docVIP

正常兔双眼视网膜电图对比及视网膜缺血-再灌注模型建立 　 作者：丁建光，姜德咏，吴荣瀚，唐朝珍，李文生 【摘要】 目的 探讨正常兔双眼ERG有无差异，建立兔视网膜缺血-再灌注的血管结扎模型。方法 20只兔，双眼均行ERG测定后随机分成A、B两组，A组兔10只，均右眼暴露视神经后不结扎。B组兔10只，均左眼视神经结扎60min。分别测定再灌注0h，3h，24h，48h，168h ERG a、b波振幅(aA、bA)值。结果 (1)正常兔左、右眼ERG aA、bA值差异无显著性，但个体之间存在一定差异。(2)未结扎组视网膜缺血-再灌注0h，3h，24h，48h，168h的ERG aA、bA值差异无显著性。(3)结扎后兔ERG aA、bA值均明显下降，再灌注24h最低，后逐渐上升。结论 (1)正常兔双眼ERG差异无显著性，但不同个体间有较大差异。(2)成功地建立了兔视网膜缺血-再灌注的血管结扎模型，发现缺血60min，再灌注24h时视网膜功能受到进一步损伤。 【关键词】 视网膜 缺血-再灌注损伤 视网膜电图 兔 Comparison of ERG in both normal eyes and establishment of experimental retinal ischemia-reperfusion injury model in rabbits 【Abstract】 Objective To compare electroretinogram(ERG)in both normal eyes and establish a retinal ischemia-reperfusion injury model in rabbits.Methods 20 healthy rabbits were randomly divided into two groups(A，B)after ERG of both eyes.The retinal ischemia in the left eye of 10 rabbits in group B was induced by temporary ligation of the optic nerve for 60min，and a sham procedure was performed on the right eye of 10 rabbits in group A.The amplitudes of a，b-wave of(aA，bA)ERG were recorded at 0，3，24，48，168h of reperfusion.Results (1)No significant difference was found in aA，bA of ERG between left and right eye in normal rabbits，but difference existed in individuals.(2)No significant difference was found in aA，bA of ERG at 0，3，24，48，168h of reperfusion in rabbits served as a non-ischemia control.(3)Both aA and bA were significantly decreased after a ligation.The aA，bA of ERG were decreased to the bottom at 24h of reperfusion，then gradually increased in the following time.Conclusion (1)No significant difference is found in ERG between left and right eye in normal rabbits，but difference exists in individuals.(2)A retinal is chemia-reperfusion injury model in rabbits is established successfully.The retinal function aggravated is found at 24h of reperfusion after 60min ischemia. 【Key words】 retina ischemia-reperfusion injury electroretinogram rabbit 视网膜缺血-再灌注损伤是目前研究的一个热点，由于视网膜缺血-再灌注损伤的机制较为复杂，因此，通过