牛大力多糖对小鼠T淋巴细胞增殖双向调节作用.docVIP

牛大力多糖对小鼠T淋巴细胞增殖双向调节作用 　 ; 作者：郑元升，蒲含林，麻建军 【摘要】; 目的 从牛大力(Millettia Speciosa Champ.)提取纯化多糖，流式细胞术检测多糖对刀豆蛋白A(Con A)刺激引起的小鼠T淋巴细胞增殖的影响，探讨牛大力多糖对免疫系统的调节作用机制，为临床用牛大力治疗多种慢性疾病提供理论和实验依据。方法 提取、纯化牛大力多糖，利用CFDA-SE染色,流式细胞术检测多糖对Con A刺激引起的小鼠T淋巴细胞增殖的影响。结果 终浓度为160 μg/mL、 800 μg/mL、4 mg/mL、20 mg/mL的多糖对小鼠T淋巴细胞增殖呈剂量依赖性抑制,而终浓度为5、25、50、125 μg/mL的多糖对小鼠T淋巴细胞增殖起促进作用,剂量依赖关系不明显。结论 牛大力多糖对小鼠T淋巴细胞的增殖呈双向调节作用。 【关键词】; 牛大力；多糖；T淋巴细胞；CFDA-SE 　　Abstract:Objective The polysaccharide was extracted and purified from Millettia Speciosa Champ. Flow cytometry was used to investigated the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A,the mechanism of the effects of polysaccharide on the immune system was explored and theoretical and experimental evidence for the clinical application of Millettia Speciosa Champ in treating chronic diseases were drawn.Methods; The polysaccharide was extracted and purified from Millettia Speciosa Champ,CFDA-SE staining and flow cytometry were used to detect the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A.Results; The polysaccharide has the function to inhibit the proliferation of lymphocyte of mouse depending on its concentration with 160 μg/mL,800 μg/mL,4 mg/mL,20 mg/mL in substrate,but promotes the proliferation of T lymphocytes of mice lymph node at the concentrations of 5,25,50,and 125 μg/mL,,but the dose-dependent effect is not significant.Conclusion The polysaccharide extracted from Millettia Speciosa Champ has the two-way effects on T lymphocyte proliferation in mice lymph node stimulated by Con A. 　　Key words:Millettia Speciosa Champ.; polysaccharide; T lymphocyte; CFDA-SE 　　牛大力(Millettia Speciosa; Champ.)又叫山莲藕、大力薯，豆科崖豆藤属植物美丽崖豆藤(Millettia speciosa; Champ.)的干燥根［1］，主产于广东东部。牛大力用药始载于《生草药性备要》［2］，历史悠久，性味甘、平，具有补虚润肺、强筋活络的功用，临床主要用来治疗肺热咳嗽、风湿性关节炎、腰肌劳损、胃溃疡、慢性肝炎、肺结核等疾病［1］。早在20世纪70年代，牛大力作为壮腰健肾丸、强力健身胶囊的原料已用于中成药的生产［3］。目前，对牛大力免疫活性的研究有过报道［4］，但对其多糖药理活性尚未见报道。本文提取、分离、纯化牛大力多糖，鉴定多糖主要的理化性质，并利用CFDA-SE染色、流式细胞术研究多糖对刀豆蛋白A(Con A)引起的T