荧光定量PCR检测乙型肝炎临床探究.docVIP

荧光定量PCR检测乙型肝炎临床探究 　【关键词】 FQ 　　［摘 要］ 目的：探讨荧光定量聚合酶链反应(FQPCR)方法检测乙型肝炎的临床价值。方法：应用FQPCR技术对1 562例乙型肝炎阳性血清标本和无症状健康者血清进行HBVDNA检测并对不同血清型的HBVDNA进行比较。结果：经FQPCR检测,380例HBsAg(+)、HBeAg(+)、HBcAb(+)其HBVDNA阳性率100%；246例HBsAg(+)、HBeAb(+)、HBcAb(+)其HBVDNA阳性率88.6%；210例HBsAg(+)、HBeAg(+)其HBVDNA阳性率98.1%；190例HBsAg(+)、HBcAb(+)其HBVDNA阳性率69.5%；146例HBsAb(+)、HBeAb(+)、HBcAb(+)其HBVDNA阳性率22.6%；66例HBsAb(+)其HBVDNA阳性率6.1%；20例HBsAb(+)、HBcAb(+)其HBVDNA阳性率60.0%；18例HBsAb(+)、HBeAb(+)其HBVDNA阳性率55.5%；286例“两对半”五项全阴性的病例中有4例HBVDNA阳性,其阳性率为1.4%。结论：血清中HBVDNA水平反映了体内HBV是否复制及复制的程度，定量PCR是鉴别HBV感染各期有价值的工具，是评价传染性的可靠方法。定量PCR测定灵敏度高，对乙型肝炎的临床诊断、治疗方案的选择和疗效观察有重要意义。 　　［关键词］ FQPCR；HBVDNA；乙型肝炎病毒 　　Clinical Researchon Detection of Hepatitis Bvirusby Fluorescence Quatitative PCR 　　Abstract：Objective To set up aFluorescence QuatitativePCR assay to explore the clinical value of detecting the load of HBVDNA.Methods FQPCR technique was used to measure 1562 samples HBVDNA of thedifferent cases of hepatitis B and the cases of HBV antigen or antibodynegativeand tocomparewithits.Results Using ELISA as contrast,in 1562 clinical serum samples were tested.380 HBsAg(+)、HBeAg(+)、HBcAb(+) samples,FQPCR were all positive,with 1.21×106～7.69×109 CP/mL.246 HBsAg(+)、HBeAb(+)、HBcAb(+) samples,positive rate of 88.6%,with 1.01×104～9.08×106 CP/mL.210 HBsAg(+)、HBeAg(+) samples 98.1%,190 HBsAg(+)、HBcAb(+) samples 69.5%,286 with ELISA five indexes all negative samples there were4 HBVDNA positive,1.4%.66 HBsAb(+) samples 6.1%.146 HBsAb(+)、HBeAb(+)、HBcAb(+) samples 22.6%.20 HBsAb(+)、HBcAb(+) samples 60%.18 HBsAb(+)、 HBeAb(+) samples55.5%.Conclusion FQPCR was an accurate quatitative technique in detecting HBVDNA and it was significant better than ELISA technique in diagnosis of hepatitis B and selection of antivirus drugs.FQPCR can be used to minitor the true state of HBV infection and amplification.It was importanttoclinical diagnoseand selecttreatmentandminitor forHBV. 　　Key words：FQPCR；HBVDNA；Hepatitis B Virus 荧光定量PCR在保留PCR灵敏和特异的基础上进一步利用了分子杂交技术的特异性和光谱分析技术的灵敏性