HPLC法测定种牛黄中胆红素的含量迪马科技.PDFVIP

2010 3 20 3 # # HPLC 3 , , , , , , (, 2710 16) : , 3 : D iam onsil C ( 250 mm @ 4. 6 mm, 5Lm ) 18 ; : - - 1% ( 65B32B3), : 1. 0 m l/m in; 455 nm 2. 32~ 27. 84 L /m l , A = 19 169C - 4886. 9( r = 0. 9998); 99. 38% , ( RSD) = 2. 16% , : ; ; ; ; ; 中图分类号: R282. 76 文献标志码: A 文章编号: 1673- 46 10( 2010) 03- 0017- 03 Determ ination Content ofBilirubin in 3 Kinds of CalculusBovis by HPLC LI K e, Q IYon -x iu, GAO Yun-shen , CHEN M in -x in , LIYuq in, JIA Bao-x iu, LIU Ca-i hon (D epartment of Pharmacy, Taishan M ed icalColle e, Taian, Shandon 271016, Ch ina) A b s tra c t: O b je c t iv e To estab lish aHPLC method for determ ination of b ilirubin, and to compare the conten t of 3 kinds of bezoar. M e th o d s The C ( 250 mm @ 4. 6 mm, 5 Lm) columnw as used. The mob ile phase w asM ethano-l 18 ch loroform-1% acetic acid ( 65B32B3) . The flow rate w as 1. 0 m l/m in, and the detection w avelen th w as 455 nm. Re s u lts The bilirubinw as in a ood linear relationsh ip ( r = 0. 9998) at the concentration of 2. 32~ 27. 84 L /m .l The avera e recovery w as 99. 38% , and RSD w as 2. 16%. C o n c lu s io n Them ethod w as smi ple, accurate, specific, and can be used for the quality control of bilirub in in calcu lus bovis, succedaneum s and preparation of calcu lus bovis. K ey w o rd s: NaturalCalculus Bovis; Calculus Bovis Cu ltivated by G lucuronidase; A tificailCalcu lus Bovis; B ilirub in; Determ ination; HPLC , , 1 680 W aters 600, 2996 DAD, Em- ,